Introduction: Detection of extended-spectrum beta-lactamases (ESBLs) could be a major challenge for microbiologists--the difficulties arise mainly from the phenotypic differences among strains.
Materials And Methods: Evaluation of ESBLs was performed on 42 strains of E. coli by: 1) DDST on MHA, 2) DDST on MHA with cloxacillin, 3) CT on MHA, according to CLSI, 4) CT on MHA with cloxacillin, 5) Etest ESBL (AB Biodisk), 6) CHROMagarTM ESBL (GRASO), 7) ChromID® ESBL (bioMérieux), and 8) automatic system VITEK2 ESBL test (bioMérieux).
Result: Positive results were obtained for 20 strains using method 1, for 18 strains using method 2, 17 by method 3, 14 by method 4, 11 by method 5, 39 by method 6, 40 by method 7, and 15 by method 8. Using Etest ESBL 6.0 non-determinable results were obtained. The most consistent results were obtained when comparing the results of method 3 with results of method 2 (97.6%), and comparing the results obtained using methods 3 and 8 (95.2%).
Conclusions: Based on our study we conclude that the chromogenic media can only be used as a screening method for the detection of ESBLs in E. coli rods. Etest is less useful compared to other phenotype methods, due to the impossibility of obtaining results for all the tested strains. Adding cloxacillin to MHA does not increase the frequency of detection of ESBLs in E. coli strains. DDST seems to be the most reliable among phenotypic methods for the detection of ESBLs in E. coli rods.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.5604/17322693.1108873 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Syphilis-positive and false-positive trends among US blood donors, 2013-2023.
Transfusion
January 2025
Infectious Disease Consultant, North Potomac, Maryland, USA.
Background: US blood donors are tested for syphilis because the bacterial agent is transfusion transmissible. Here we describe trends over an 11-year period of donations positive for recent and past syphilis infections, and donations classified as syphilis false positive (FP).
Methods: Data from January 1, 2013, to December 31, 2023 (11 years) were compiled for all American Red Cross blood donations to evaluate demographics/characteristics and longitudinal trends in donors testing syphilis reactive/positive.
Validating the Accuracy of Parkinson's Disease Clinical Diagnosis: A UK Brain Bank Case-Control Study.
Ann Neurol
January 2025
Research Unit of Neurology, Neurophysiology and Neurobiology, Department of Medicine and Surgery, Università Campus Bio-Medico di Roma, Rome, Italy.
Objective: Despite diagnostic criteria refinements, Parkinson's disease (PD) clinical diagnosis still suffers from a not satisfying accuracy, with the post-mortem examination as the gold standard for diagnosis. Seminal clinicopathological series highlighted that a relevant number of patients alive-diagnosed with idiopathic PD have an alternative post-mortem diagnosis. We evaluated the diagnostic accuracy of PD comparing the in-vivo clinical diagnosis with the post-mortem diagnosis performed through the pathological examination in 2 groups.
View Article and Find Full Text PDFBridging Classrooms and Communities: The Transformative Impact of Community-Based Dental Education on the Learning Experiences of Undergraduate Students.
Eur J Dent Educ
January 2025
QU Health College of Dental Medicine, Qatar University, Doha, Qatar.
Aims: This study aimed to evaluate the impact of community-based dental education (CBDE) on the learning experiences of undergraduate dental students and recent dental graduates from two diverse geographical regions.
Methods: The study followed a cross-sectional design, conducted online using Google Forms, with ethical approval from Qatar University. A non-probability purposive sampling method was used to recruit dental students and recent graduates from three institutions in India and one in Qatar.
Manipulating Dynamic Light-Driven Solid-Liquid Transition and Static Reversible Photochromism via Organic Cocrystal Strategy.
Angew Chem Int Ed Engl
January 2025
Henan University of Technology, School of Chemistry and Chemical Engineering, CHINA.
Developing of molecular crystalline materials with light-induced multiple dynamic deformation in space dimension and photochromism on time scales has attracted much attention for its potential applications in actuators, sensoring and information storage. Nevertheless, organic crystals capable of both photoinduced dynamic effects and static color change are rare, particularly for multi-component cocrystals system. In this study, we first report the construction of charge transfer co-crystals allows their light-induced solid-to-liquid transition and photochromic behaviors to be controlled by trans-stilbene (TSB) as an electron donor and 3,4,5,6-Tetrafluorophthalonitrile (TFP) as an electron acceptor.
View Article and Find Full Text PDFT-2 Toxin Nephrotoxicity: Toxic Effects, Mechanisms, Mitigations, and Future Perspectives.
J Agric Food Chem
January 2025
College of Veterinary Medicine, Henan Agricultural University, Zhengzhou 450046, China.
T-2 toxin is a highly toxic fungal toxin that threatens humans and animals' health. As a major detoxifying and metabolic organ, the kidney is also a target of T-2 toxin. This article reviews T-2 toxin nephrotoxicity research progress, covering renal structure and function damage, nephrotoxicity mechanisms, and detoxification methods to future research directions.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!