AI Article Synopsis
- Tetrazolium salts, like tetrazolium violet, are commonly used in microbiology for staining bacteria since they produce a colored formazan product when reduced by bacteria.
- However, these salts can be toxic to bacteria, especially Salmonella, at high concentrations, affecting their growth and the staining process.
- The study found that adding magnesium to the growth medium with tetrazolium violet not only enhances the growth rate of Salmonella but also allows for growth at higher toxic concentrations of the salt, potentially improving detection methods for bacteria.
Article Abstract
Tetrazolium salts (TTZ), such as tetrazolium violet (TV), have been widely used for microbiological studies. The formation of the colored formazan product due to bacterial reduction of the uncolored reagent is extensively exploited to stain cells or colonies in agar or on filters. But an important toxic effect of tetrazolium salts on bacteria exists that limits their use at high concentrations, impairing the efficient staining of the colonies. This is especially the case for Salmonella spp. where we observed, using a classic photometric approach and mathematical modeling of the growth, an important impact of tetrazolium violet on the apparent growth rate below the inhibitory concentration. In this study, we demonstrate that adding magnesium to the medium in the presence of TV leads to a significant increase in the apparent growth rate. Moreover, when higher TV concentrations are used which lead to total inhibition of Salmonella strains, magnesium addition to the culture media allows growth and TV reduction. This effect of magnesium may allow the use of higher TTZ concentrations in liquid growth media and enhance bacteria detection capabilities.
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