AI Article Synopsis
- Squamosa-promoter binding protein (SBP)-box genes are important for plant development and some are regulated by the microRNA miR156.
- The study focused on cloning the tae-MIR156 precursor gene, revealing that several wheat SBP-box genes are influenced by this microRNA, particularly TaSPL3 and TaSPL17.
- The analysis showed that different species related to the common wheat possess multiple copies of the SPL17 gene, indicating a high level of sequence conservation and a shared evolutionary origin among these genes.
Article Abstract
Squamosa-promoter binding protein (SBP)-box genes, encoding plant-specific transcription factors, play an important role in plant development. Some members of the SBP-box gene family are regulated by miR156. In this study, we cloned the tae-MIR156 precursor gene, which could form a stem loop after its transcription. Sequence analysis showed that TaSPL3 and TaSPL17 had putative targets of tae-miR156 among the ten wheat SBP-box genes. The diploid donor species of hexaploid common wheat (Triticum aestivum, genome AABBDD), i.e., Triticum urartu (AA) UR209 and Aegilops speltoides Y2001 (SS, closely related to BB) possessed more than one copy of SPL17 (SPL17-A1, SPL17-A2 and SPL17-A3 from Triticum urartu; SPL17-B1, SPL17-B2 and SPL17-B3 from Aegilops speltoides), while Aegilops tauschii (DD) Ae38 only possessed one (SPL17-D). The identities between nucleotide sequences of SPL17-A2 and SPL17-B2, SPL17-A3 and SPL17-B3 or SPL17-D were higher than 99%. They were highly similar with the sequence of TaSPL17 in common wheat cultivars Chinese Spring, Hengguan 35 and Shuangfengshou. These genes might originate from a common ancestor and were highly conserved in the process of evolution. The target site of tae-miR156 in TaSPL17 was also highly conserved in two subgroups consisted of accessions with diverse spike number per plant and genetic background.
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| http://dx.doi.org/10.3724/SP.J.1005.2014.0592 | DOI Listing |
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Article Synopsis
- Squamosa-promoter binding protein (SBP)-box genes are important for plant development and some are regulated by the microRNA miR156.
- The study focused on cloning the tae-MIR156 precursor gene, revealing that several wheat SBP-box genes are influenced by this microRNA, particularly TaSPL3 and TaSPL17.
- The analysis showed that different species related to the common wheat possess multiple copies of the SPL17 gene, indicating a high level of sequence conservation and a shared evolutionary origin among these genes.
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