AI Article Synopsis
- miR-142-3p and miR-494 were studied for their role in cell communication and effects on the clock gene Bmal1, showing that their overexpression lowers BMAL1 levels while altering Per2 oscillation periods.
- The presence of miRNA-enriched medium boosts miR-142-3p inside cells and inhibits Bmal1 activity, suggesting they play a significant role in regulating circadian rhythms.
- Inhibitors that disrupt vesicle trafficking also affect how these miRNAs communicate between cells, further influencing synchronized circadian clock activity.
Article Abstract
Based on their extracellular expression and targeting of the clock gene Bmal1, miR-142-3p and miR-494 were analyzed for evidence of vesicle-mediated communication between cells and intracellular functional activity. Our studies demonstrate that: miR-142-3p+miR-494 overexpression decreases endogenous BMAL1 levels, increases the period of Per2 oscillations, and increases extracellular miR-142-3p/miR-494 abundance in conditioned medium; miRNA-enriched medium increases intracellular expression of miR-142-3p and represses Bmal1 3'-UTR activity in naïve cells; and inhibitors of vesicular trafficking modulate intercellular communication of these miRNAs and ensemble Per2 rhythms. Thus, miR-142-3p and miR-494 may function as cis- and trans-acting signals contributing to local temporal coordination of cell-autonomous circadian clocks.
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| http://dx.doi.org/10.1016/j.febslet.2014.05.058 | DOI Listing |
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