In human medicine, procalcitonin (PCT) is a very common and well-established biomarker for sepsis. Even though sepsis is also a leading cause of death in foals and adult horses, up to now, no data about the role of equine PCT in septic horses has been available. Based on monoclonal antibodies targeted against human PCT, we report here the development of a sandwich ELISA for the quantification of equine PCT in equine plasma samples. The ELISA was characterized for intra- and interassay variance and a working range from 25 to 1,000 ng mL(-1) was defined as within this range; both intra- and interassay variances were below 15 %. The target recovery ranged between 73 and 106 %. The ELISA was used to determine the equine PCT concentration in 24 healthy and 5 septic horses to show the potential for clinical evaluation of equine PCT. Significantly different (P = 0.0006) mean equine PCT concentrations were found for the healthy control group and the sepsis group (47 and 8,450 ng mL(-1)).
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Serum procalcitonin as a diagnostic biomarker in dogs with bacterial respiratory diseases.
Vet Clin Pathol
June 2024
Department of Equine and Small Animal Medicine, Faculty of Veterinary Medicine, University of Helsinki, Helsinki, Finland.
Background: Procalcitonin (PCT) is a useful biomarker in humans in the identification of bacterial respiratory infections.
Objectives: The aim of this study was to investigate the utility of serum PCT measurements as a diagnostic biomarker in canine bacterial lower respiratory tract diseases.
Methods: PCT concentrations were measured in serum samples with an ELISA method previously validated for dogs.
Comparison of first, second, and third versus the average of six probe-corneal touches for intraocular measurement of two rebound tonometers in healthy horses.
J Equine Vet Sci
May 2024
Department of Surgery, Faculty of Veterinary Medicine, Atatürk University, Erzurum, Turkey 25000.
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- The study aimed to assess intraocular pressure (IOP) in horses by comparing measurements from the first three probe-cornea touches (PCTs) to the average of six PCTs using two types of rebound tonometers.
- A total of 38 horses (24 Arabians and 14 cross-breeds) were tested, and the differences in IOP measurements from individual PCTs were generally small when compared to the average of six PCTs.
- Results indicated that the first PCT showed the best alignment with the average IOP readings, suggesting it could be a viable alternative for IOP measurement in horses when averaging multiple readings isn't possible.
Immunogenic profile of a plant-produced nonavalent African horse sickness viral protein 2 (VP2) vaccine in IFNAR-/- mice.
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Council for Scientific and Industrial Research (CSIR), Chemical Cluster, Pretoria, South Africa.
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Procalcitonin and carbonylated protein concentrations in equine synovial fluid.
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Department of Veterinary Sciences, Veterinary Teaching Hospital, University of Pisa, Pisa, Italy.
Early diagnosis of joint diseases is fundamental for prompt and appropriate management, particularly in septic arthritis. Procalcitonin (PCT) and protein carbonylated content (PCC) have been investigated in both human and veterinary medicine. An increase in PCT has been shown in infectious bacterial diseases, while higher levels of PCC have been shown in inflammatory pathologies characterized by oxidative damage.
View Article and Find Full Text PDFMolecularly imprinted polymers as effective capturing receptors in a pseudo-ELISA immunoassay for procalcitonin detection in veterinary species.
Anal Methods
December 2022
Department of Veterinary Science, University of Pisa, 56122 Via Livornese, PI, Italy.
In this study, a new sandwich-type immunoenzymatic assay, based on a molecularly imprinted polymer (MIP) as an artificial antibody (pseudo-ELISA), was developed for the determination of procalcitonin (PCT) in veterinary species. The quantification of PCT in human medicine represents the state of the art for the diagnosis of sepsis; instead the clinical studies on the relevance of PCT as a sepsis predictor in veterinary patients are few, likely due to the total absence of validated assays. MIPs have been widely used as antibody mimics for important applications, and MIP-based sandwich assays have emerged as promising analytical tools for the detection of disease biomarkers.
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