Differentiation of many immune-related cells is controlled by the expression levels and the activation status of transcription factors (TFs). We here describe a method to identify candidate TFs activated during the development of thymic epithelial cells (TECs) in the embryo. RNAs are isolated from fetal thymic organ cultures of wild-type and mutant mice and are subsequently analyzed by using a combination of comprehensive expression analysis and in silico data analysis in order to predict the TFs that might be activated.
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