Porous gelatin (GEL) cryogels were processed by spatiotemporal and temperature-controlled gelation and freezing-lyophilizaton process, followed by zero-length crosslinking, using different molarities of reagents (EDC and NHS) and reaction media (100% PBS or 20/80% PBS/EtOH mixture) for variable time extensions (1-24 h). In this way, tuneable cryogels with gradient microporosity (from 100 µm to 1000 µm) were formed, being mainly influenced by crosslinkers' concentration and EtOH addition. Later affect the pore morphology (from round to ellipsoid), consequently modulating the steady-state physiological swelling profile toward twice lower values (∼ 600%) comparing to stepwise swelling of in 100% PBS media crosslinked cryogels. While the presence of EtOH decelerate the crosslinking kinetic by retaining cryogels' microstructure formed during freezing, the 100% PBS and higher EDC molarity resulted in approximately 40% crosslinking degree, being expressed as a thermal resistance of cryogels up to approximately 73°C. Finally, the tuneable enzymatic resistance allow time-dependent poly-L-Lysine (pL) release profile in up to month period. The processed GEL cryogels have potential in broad range biomedical applications, especially as sustainable, protein-based drug delivery systems.
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http://dx.doi.org/10.1002/jbm.a.35261 | DOI Listing |
Mar Biotechnol (NY)
January 2025
Department of Aquatic Life Medicine, Pukyong National University, Busan, 48513, South Korea.
Vibriosis caused by Vibrio anguillarum has been an important bacterial disease in cultured rainbow trout (Oncorhynchus mykiss). In the present study, we evaluated the protective efficacy of a vaccine that consists of formalin-killed (FK) V. anguillarum and the alr genes knockout auxotrophic-live (AL) V.
View Article and Find Full Text PDFPharmaceutics
January 2025
Department of Mathematical and Physical Sciences, Catholic University of Temuco, Temuco 4813302, Chile.
: A previous study investigated the in vitro release of methylene blue (MB), a widely used cationic dye in biomedical applications, from nanocellulose/nanoporous silicon (NC/nPSi) composites under conditions simulating body fluids. The results showed that MB release rates varied significantly with the nPSi concentration in the composite, highlighting its potential for controlled drug delivery. To further analyze the relationship between diffusion dynamics and the MB concentration, this study developed a finite element (FE) method to solve Fick's equations governing the drug delivery system.
View Article and Find Full Text PDFPharmaceutics
January 2025
Laboratory of Nuclear Medicine (LIM-43), Hospital das Clinicas HCFMUSP, Faculdade de Medicina, Universidade de Sao Paulo, Sao Paulo 05403-911, SP, Brazil.
Background/objectives: Dithiocarbazates (DTCs) and their metal complexes have been studied regarding their property as anticancer activities. In this work, using S-benzyl-5-hydroxy-3-methyl-5-phenyl-4,5-dihydro-1H-pirazol-1-carbodithionate (Hbdtc), we prepared [ReO(bdtc)(Hbdtc)] and [[Tc]TcO(bdtc)(Hbdtc)] complexes for tumor uptake and animal biodistribution studies.
Methods: Re complex was prepared by a reaction of H2bdtc and (NBu)[ReOCl], the final product was characterized by IR, H NMR, CHN, and MS-ESI.
Sensors (Basel)
January 2025
Microbiology Institute of Shaanxi, No.76 Xiying Road, Xi'an 710043, China.
The trace detection of pyocyanin (PCN) is crucial for infection control, and electrochemical sensing technology holds strong potential for application in this field. A pivotal challenge in utilizing carbon materials within electrochemical sensors lies in constructing carbon-based films with robust adhesion. To address this issue, a novel composite hydrogel consisting of multi-walled carbon nanotubes/polyvinyl alcohol/phosphotungstic acid (MWCNTs/PVA/PTA) was proposed in this study, resulting in the preparation of a highly sensitive and stable PCN electrochemical sensor.
View Article and Find Full Text PDFMaterials (Basel)
January 2025
Chair and Department of Biochemistry and Biotechnology, Medical University of Lublin, Chodzki 1 Street, 20-093 Lublin, Poland.
The present article focuses on the characterization of the new biocomposites of poly(butylene succinate) (PBS) with fillers of plant origin such as onion peels (OP) and durum wheat bran WB () subjected to composting and artificial aging. The susceptibility to fungal growth, cytotoxicity and antibacterial properties were also examined. The biodegradation of the samples was investigated under normalized conditions simulating an intensive aerobic composting process.
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