Introduction: The optimization of an organic scaffold for specific types of applications and cells is vital to successful tissue engineering. In this study, we investigated the effects of a new fibrin sealant derived from snake venom as a scaffold for mesenchymal stem cells, to demonstrate the ability of cells to affect and detect the biological microenvironment.
Methods: The characterization of CD34, CD44 and CD90 expression on mesenchymal stem cells was performed by flow cytometry. In vitro growth and cell viability were evaluated by light and electron microscopy. Differentiation into osteogenic, adipogenic and chondrogenic lineages was induced.
Results: The fibrin sealant did not affect cell adhesion, proliferation or differentiation and allowed the adherence and growth of mesenchymal stem cells on its surface. Hoechst 33342 and propidium iodide staining demonstrated the viability of mesenchymal stem cells in contact with the fibrin sealant and the ability of the biomaterial to maintain cell survival.
Conclusions: The new fibrin sealant is a three-dimensional scaffolding candidate that is capable of maintaining cell survival without interfering with differentiation, and might also be useful in drug delivery. Fibrin sealant has a low production cost, does not transmit infectious diseases from human blood and has properties of a suitable scaffold for stem cells because it permits the preparation of differentiated scaffolds that are suitable for every need.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4100340 | PMC |
| http://dx.doi.org/10.1186/scrt467 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Effectiveness of TachoSil as Sealant in Lymphatic Leakage of Breast Cancer With Axillary Dissection.
Int J Breast Cancer
December 2024
College of Medicine, King Faisal University, Al-Ahsa City, Eastern Province, Saudi Arabia.
This study is aimed at evaluating the effectiveness of TachoSil in controlling lymphatic leakage in breast cancer patients undergoing axillary dissection. By examining its ability to reduce postsurgical lymphatic drainage, the study will assess its impact on complications like seroma formation, recovery time, and overall patient outcomes, including quality of life and reduced healthcare costs. Breast cancer patients treated in the Department of Surgical Oncology at King Abdulaziz Medical City were enrolled to receive either TachoSil or undergo drain placement after axillary dissection.
View Article and Find Full Text PDFApplication of Mesenchymal Stem Cell-Derived Schwann Cell-like Cells Spared Neuromuscular Junctions and Enhanced Functional Recovery After Peripheral Nerve Injury.
Cells
December 2024
Department of Biochemistry, College of Medicine, Ewha Womans University, Seoul 07804, Republic of Korea.
In general, the nerve cells of the peripheral nervous system regenerate normally within a certain period after the physical damage of their axon. However, when peripheral nerves are transected by trauma or tissue extraction for cancer treatment, spontaneous nerve regeneration cannot occur. Therefore, it is necessary to perform microsurgery to connect the transected nerve directly or insert a nerve conduit to connect it.
View Article and Find Full Text PDFComparison of glued platelet-rich fibrin membrane and noneugenol pack as periodontal dressings after gingival depigmentation.
J Indian Soc Periodontol
December 2024
Department of Periodontology, K. M. Shah Dental College and Hospital, Sumandeep Vidyapeeth, Vadodara, Gujarat, India.
Article Synopsis
- Periodontal surgery often requires dressings for protection and healing, with common options like noneugenol packs having drawbacks such as plaque buildup and minimal healing benefits.
- Platelet-rich fibrin (PRF) membranes promote faster healing due to their growth factors but typically require sutures for stability.
- This case report explores the use of PRF membranes combined with N-butyl cyanoacrylate adhesive as a promising alternative to traditional dressings, potentially eliminating the need for suturing.
Robot-Assisted Laparoscopic Application of Fibrin Sealant Patch for Repair of Inferior Vena Cava.
CRSLS
January 2025
Northwell Health-Lenox Hill Hospital, New York, NY. (Drs. Chu, Alden, and Seckin).
Introduction: There is a risk of iatrogenic vascular injuries during robotic-assisted laparoscopic excision of diaphragmatic endometriosis. Although studies are limited, the first reported case of a suprahepatic inferior vena cava (IVC) injury during robotic diaphragmatic endometriosis excision was successfully treated using a fibrin sealant patch, preventing exsanguination and conversion to laparotomy.
Case Description: A 36-year-old female with a history of recurrent catamenial pneumothorax and two prior video-assisted thoracoscopic surgeries to treat diaphragmatic endometriosis presented to our clinic with right-sided shoulder pain and a chest tube in place.
Role of CT myelography in the diagnosis and management of spontaneous intracranial hypotension.
Clin Neurol Neurosurg
December 2024
CHU de Lille, 2 Avenue Oscar Lambret, Hauts-de-France, France. Electronic address:
Introduction: Spontaneous intracranial hypotension (SIH) is a secondary cause of headache. Its pathophysiology is complex, and relies mainly on the notion of a localized leak, and thus a loss of cerebrospinal fluid (CSF) in the spinal region. SIH is little known to the medical profession, for which CT myelography is a technique on the rise, allowing to identify and treat the leak using a blood-patch or a fibrin sealant.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!