Yeast two-hybrid system for dissecting the rice MAPK interactome.

Methods Mol Biol

Department of Molecular Biology, College of Life Sciences, Sejong University, 98 Gunja-dong, Gwangjin-gu, Seoul, 143-747, Republic of Korea.

Published: January 2015

Protein-protein interactions are a preliminary but fundamental key to many biological systems. Identification of proteins that interact with particular bait not only contributes to a deeper understanding of bait protein function but also provides much information for the discovery of larger-scale interaction networks (interactome). Therefore, protein-protein interaction mapping is regarded as a widely accepted standardized functional genomics technique that provides comprehensive functional interpretation of previously uncharacterized proteins. A commonly used approach to detecting novel protein-protein interactions is the yeast two-hybrid system. In this chapter we describe in detail the protocols used to dissect the rice MAPK interactome, including the bait protein auto-activation test, identification of a rice MAPK interacting protein, confirmation of interaction by retransformation assay and characterization of the novel interacting protein.

Download full-text PDF

Source
http://dx.doi.org/10.1007/978-1-4939-0922-3_16DOI Listing

Publication Analysis

Top Keywords

rice mapk
12
yeast two-hybrid
8
two-hybrid system
8
mapk interactome
8
interactome protein-protein
8
protein-protein interactions
8
bait protein
8
interacting protein
8
system dissecting
4
dissecting rice
4

Similar Publications

Want AI Summaries of new PubMed Abstracts delivered to your In-box?

Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!