A column ion-exchange chromatographic method for the determination of 2-carboxy thiazolidine-4-carboxylic acid (TDCA) in blood and urine is described. After elimination of endogenous thiols, alkaline hydrolysis of the compound yields cysteine which is determined spectrophotometrically. The described method is specific for intact TDCA. Using a 3-ml aliquot of sample it allows the linear determination of this hepatoprotective drug from 5 micrograms TDCA ml-1 with a detection limit of 3 micrograms ml-1. The method was applied to study the time course of plasma levels and urinary excretion. An in vitro metabolism study showed that TDCA has a potential as a cysteine donor for glutathione synthesis.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/0731-7085(89)80116-5 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Novel A-π-D-A organic dyes for better photovoltaic performance.
RSC Adv
September 2024
Nanomaterials and Solar Energy Conversion Laboratory, Department of Chemistry, National Institute of Technology Trichy 620015 India
In this article, we report two indole-based metal-free organic dyes In-T-2C (3-(5-(3-((2-carboxy-2-cyanovinyl)-1-pentyl-1-indol-5-yl)thiophen-2-yl)-2-isocyanoacrylic acid) and In-B-C (2-cyano-3-(5-(4-cyanophenyl)-1-pentyl-1-indol-3-yl)acrylic acid) with A-π-D-A architecture. The molecular structures of metal-free indole-based A-π-D-A organic dyes were elucidated using FT-IR, NMR, HRMS and single-crystal X-ray diffraction techniques. The present investigation examined the features of the synthesized dyes employing photophysical attributes, electrochemical traits and theoretical studies were executed to acquire a detailed comprehension of the geometry, electronic structure and absorption spectra of the synthesized dyes using density functional theory (DFT) and time-dependent density functional theory (TD-DFT).
View Article and Find Full Text PDFVirtual screening and molecular dynamics simulations of phytochemicals targeting cofactor-independent phosphoglycerate mutase in antimicrobial-resistant .
3 Biotech
October 2024
Department of Bioinformatics, Central University of South Bihar, Gaya, 824236 India.
poses a significant challenge in clinical treatment due to its increasing antimicrobial resistance. This study investigates alternative therapeutic approaches by targeting the cofactor-independent phosphoglycerate mutase (iPGM) enzyme with phytochemicals derived from ethnobotanical plants. In silico screening identified several promising inhibitors, with 2-carboxy-D-arabinitol demonstrating the highest binding affinity (- 9.
View Article and Find Full Text PDFRegulation of Rubisco activity by interaction with chloroplast metabolites.
Biochem J
August 2024
Lancaster Environment Centre, Lancaster University, Lancaster LA1 4YQ, U.K.
Rubisco activity is highly regulated and frequently limits carbon assimilation in crop plants. In the chloroplast, various metabolites can inhibit or modulate Rubisco activity by binding to its catalytic or allosteric sites, but this regulation is complex and still poorly understood. Using rice Rubisco, we characterised the impact of various chloroplast metabolites which could interact with Rubisco and modulate its activity, including photorespiratory intermediates, carbohydrates, amino acids; as well as specific sugar-phosphates known to inhibit Rubisco activity - CABP (2-carboxy-d-arabinitol 1,5-bisphosphate) and CA1P (2-carboxy-d-arabinitol 1-phosphate) through in vitro enzymatic assays and molecular docking analysis.
View Article and Find Full Text PDFMolecular rotor as an in-situ fluorescent probe for the degree of polymerization of α-D-1,4-glucans.
Carbohydr Polym
January 2024
Beijing Key Laboratory of Plant Protein and Cereal Processing, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China. Electronic address:
Various starch synthesis and tailoring processes involve prevailing adjustments in the degree of polymerization (DP) of linear α-D-1,4-glucan chains (LGCs) for the improved functional performances. Previous studies indicated that LGCs might hinder the twisted relaxation of 9-(2-carboxy-2-cyanovinyl)-julolidine (CCVJ, a hydrophilic molecular rotor), highlighting CCVJ as a potential in-situ structural probe for LGC. In this study, glucose and its α-D-1,4 oligomers and polymers with molecular weights ranging from 0.
View Article and Find Full Text PDF2-[()-(2-carboxybenzylidene) amino] ethan ammonium-like amino acid zwitterions: crystal structure, functional studies and its molecular dynamic simulation study with drug target receptors.
J Biomol Struct Dyn
August 2024
Department of Biotechnology, KITS, Coimbatore, Tamil Nadu, India.
The novel synthetic amino acid-like zwitterion containing imine bond ionic compound 2-[()-(2-carboxy benzylidene) amino] ethan ammonium salt, CHNO was synthesized. Computational functional characterization is now being used to predict novel compounds. Here, we report on a titled combination that has been crystallizing in orthorhombic space group Pcc2 with = 4.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!