AI Article Synopsis
- A new conotoxin called BnIIID was isolated and characterized from the venom of the Conus bandanus snail found in Vietnam.
- The peptide has a sequence of 15 amino acids, including six cysteines and three specific post-translational modifications (PTMs): bromotryptophan, γ-carboxy glutamate, and amidated aspartic acid.
- BnIIID is classified in the M-1 family of conotoxins, making it the first known member of the M-superfamily to contain bromotryptophan as a PTM.
Article Abstract
A novel conotoxin (conopeptide) was biochemically characterized from the crude venom of the molluscivorous marine snail, Conus bandanus (Hwass in Bruguière, 1792), collected in the south-central coast of Vietnam. The peptide was identified by screening bromotryptophan from chromatographic fractions of the crude venom. Tandem mass spectrometry techniques were used to detect and localize different post-translational modifications (PTMs) present in the BnIIID conopeptide. The sequence was confirmed by Edman's degradation and mass spectrometry revealing that the purified BnIIID conopeptide had 15 amino acid residues, with six cysteines at positions 1, 2, 7, 11, 13, and 14, and three PTMs: bromotryptophan, γ-carboxy glutamate, and amidated aspartic acid, at positions "4", "5", and "15", respectively. The BnIIID peptide was synthesized for comparison with the native peptide. Homology comparison with conopeptides having the III-cysteine framework (-CCx1x2x3x4Cx1x2x3Cx1CC-) revealed that BnIIID belongs to the M-1 family of conotoxins. This is the first report of a member of the M-superfamily containing bromotryptophan as PTM.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4071585 | PMC |
| http://dx.doi.org/10.3390/md12063449 | DOI Listing |
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Article Synopsis
- A new conotoxin called BnIIID was isolated and characterized from the venom of the Conus bandanus snail found in Vietnam.
- The peptide has a sequence of 15 amino acids, including six cysteines and three specific post-translational modifications (PTMs): bromotryptophan, γ-carboxy glutamate, and amidated aspartic acid.
- BnIIID is classified in the M-1 family of conotoxins, making it the first known member of the M-superfamily to contain bromotryptophan as a PTM.
High accuracy mass spectrometry comparison of Conus bandanus and Conus marmoreus venoms from the South Central Coast of Vietnam.
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December 2013
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Cone snail (genus Conus) venoms provide a rich source of small bioactive peptides known as conopeptides or conotoxins, which are highly interesting in pharmacological studies for new drug discovery. Conus species have evolved expressing a variety of conopeptides, adapted to the biological targets of their own specific preys at their living environments. Therefore, the potential proteomic evaluation of Conus venom components, poorly studied, is of great interest.
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