Objective: To determine whether there is an association between KRAS status and changes in nuclear morphometric properties.
Study Design: A total of 60 cases were included in the analysis. Thirty cases carried a mutated KRAS oncogene, while 30 bore a wild-type KRAS gene. Computerized morphometry was used to measure various nuclear indices of size, shape, and texture.
Results: Nuclear ellipticity (p = 0.02) and chromatin textural contrast (p = 0.02) were both significantly higher in the mutated KRAS group. However, chromatin contrast was the only independent predictor of KRAS status. A ROC analysis detected a best chromatin contrast cutoff point of 798, with a sensitivity of 89.7% and a specificity of 52%. Tumor grade was also associated with nuclear ellipticity (p = 0.001). Yet, no association was found between tumor grade and KRAS status.
Conclusion: Our results suggest that the presence of a KRAS mutation in colorectal carcinoma is associated with alterations in the nuclear morphometric properties of contrast and ellipticity.
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