AI Article Synopsis
- The study confirmed the presence of ADM using various techniques and investigated its effect on chloride secretion in mouse seminal vesicles through experiments involving specialized chambers and receptor antagonists.
- Results indicated that ADM increases chloride secretion via calcium-activated chloride channels, involving calcitonin gene-related peptide (CGRP) receptors, suggesting it could impact the composition of seminal plasma and sperm health.
Article Abstract
Adrenomedullin (ADM) may regulate seminal vesicle fluid secretion, and this may affect sperm quality. In this study, we investigated the effect of ADM on chloride secretion in the mouse seminal vesicle. The presence of ADM in mouse seminal vesicle was confirmed using immunostaining, and the molecular species was determined using gel filtration chromatography coupled with enzyme-linked assay for ADM. The effects of ADM on chloride secretion were studied by short-circuit current technique in a whole-mount preparation of mouse seminal vesicle in an Ussing chamber. The effects of specific ADM and calcitonin gene-related peptide (CGRP) receptor antagonists were investigated. Whether the ADM effect depended on the cAMP- and/or calcium-activated chloride channel was also studied using specific chloride channel blockers. The results showed that ADM was present in seminal vesicle epithelial cells. The major molecular species was precursor in the mouse seminal vesicle. ADM increased short-circuit current through the calcium-activated chloride channel in mouse seminal vesicle, and CGRP receptor was involved. We conclude that ADM may regulate chloride and fluid secretion from the seminal vesicle, which may affect the composition of the seminal plasma bathing the sperm and, hence, fertility.
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| http://dx.doi.org/10.1095/biolreprod.113.116848 | DOI Listing |
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