AI Article Synopsis
- The study applied Neospora caninum microsatellite markers to analyze clinical samples from infected zebuine foetuses in Goiás, Brazil, focusing on DNA from 15 brains and four hearts.
- Using fluorescently labeled DNA fragment analysis and sequencing, researchers obtained distinct allele profiles from the samples, discovering three different genotypes unique to the region and a new allele.
- The findings indicate that there are genetic differences in N. caninum strains affecting foetal transmission in South America, marking a significant advancement in understanding these infections.
Article Abstract
The Neospora caninum microsatellite markers were applied to clinical samples. Genotyping technology involving fluorescently labelled DNA fragment analysis was used in combination with DNA sequencing for markers with complex repetitive sequences. Nineteen DNA samples from 15 brains and four hearts of naturally infected non-aborted zebuine foetuses from abattoirs in Goiás, Brazil. N. caninum had been detected in these foetuses by nested-PCR of the internal transcribed spacer-1 rRNA region, and the samples were analysed using these microsatellites. Seven complete or nearly complete allele profiles were obtained from six foetuses. Three distinct profiles of N. caninum were identified in a unique microregion (Meia Ponte) of Goiás. Two alleles for the same marker were detected in a unique foetus that was probably infected with two different strains. A new allele for one of the microsatellites is described. The multilocus analysis performed here revealed a preliminary means of discriminating between individual strains according to their geographical origins. These are the first results that have been obtained regarding the molecular characterisation of strains of N. caninum from infected zebuine foetuses in South America and reveal for the first time that there are genotypic differences in the strains that are responsible for foetal transmission in zebuine foetuses.
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| http://dx.doi.org/10.1016/j.vetpar.2014.05.011 | DOI Listing |
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Genetic characterisation of Neospora caninum strains from clinical samples of zebuine foetuses obtained in abattoirs in Goiás, Brazil.
Vet Parasitol
August 2014
Veterinary Parasitology Laboratory/Department of Microbiology, Immunology, Parasitology and Pathology, Institute of Public Health and Tropical Pathology, Federal University of Goiás, Rua 235, s/n, Setor Universitário, CEP 74605-050, Goiânia, Goiás, Brazil. Electronic address:
Article Synopsis
- The study applied Neospora caninum microsatellite markers to analyze clinical samples from infected zebuine foetuses in Goiás, Brazil, focusing on DNA from 15 brains and four hearts.
- Using fluorescently labeled DNA fragment analysis and sequencing, researchers obtained distinct allele profiles from the samples, discovering three different genotypes unique to the region and a new allele.
- The findings indicate that there are genetic differences in N. caninum strains affecting foetal transmission in South America, marking a significant advancement in understanding these infections.
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