A rapid and simple method for the fabrication of the island patterns with particles and cells was applied to detect the presence of specific antigens on the cell surface. An upper interdigitated microband array (IDA) electrode was mounted on a lower substrate with the same design to fabricate a microfluidic-channel device for dielectrophoretic manipulation. The electrode grid structure was fabricated by rotating the upper template IDA by 90° relative to the lower IDA. A suspension of anti-CD33 modified particles and HL-60 cells was introduced into the channel. An AC electrical signal (typically 20 V peak-to-peak, 100 kHz) was then applied to the bands of the upper and lower IDAs, resulting in the formation of island patterns at the intersections with low electric fields. Immunoreactions between the antibodies immobilized on the accumulated particles and the CD33 present on the surface of the cells led to the formation of complexes comprising corresponding antigen-antibody pairs. Non-specific pairs accumulated at the intersection, which did not form complexes, were then dispersed after removal of the applied field. The time required for the detection of the formation/dispersion of the complexes is as short as 6 min in the present procedure. Furthermore, this novel cell binding assay does not require pretreatment such as target labeling or washing of the unbound cells.
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http://dx.doi.org/10.1016/j.bios.2014.05.019 | DOI Listing |
Sensors (Basel)
November 2024
Graduate School of Applied Science and Engineering, National Defense Academy, Yokosuka 239-8686, Japan.
Various types of dielectrophoresis (DEP) cell separation devices using AC electric fields have been proposed and developed. However, its capability is still limited by a lack of quantitative characterization of the relationship between frequency and force. In the present study, this limitation was addressed by developing a method capable of fast and accurate quantification of the dielectric properties of biological cells.
View Article and Find Full Text PDFElectrophoresis
November 2024
School of Molecular Sciences, Arizona State University, Tempe, Arizona, USA.
There is tantalizing evidence that proteins can be accurately and selectively manipulated by higher order electric field effects within microfluidic devices. The accurate and precise manipulation of proteins in these platforms promises to disrupt and revolutionize many fields, most notably analytical biochemistry. Several lines of experimental evidence suggest much higher forces are generated compared to those calculated from traditional theories and those higher forces arise from subtle structural features of the proteins providing selectivity.
View Article and Find Full Text PDFSensors (Basel)
November 2024
Department of Applied Physics, National Defense Academy, Hashirimizu 1-10-20, Yokosuka City 239-0802, Kanagawa, Japan.
Technologies for rapid and high-throughput separation of rare cells from large populations of other types of cells have recently attracted much attention in the field of bioengineering. Among the various cell separation technologies proposed in the past, dielectrophoresis has shown particular promise because of its preciseness of manipulation and noninvasiveness to cells. However, one drawback of dielectrophoresis devices is that their application of high voltage generates Joule heat that exposes the cells within the device to high temperatures.
View Article and Find Full Text PDFLab Chip
November 2024
Department of Civil Engineering and Computer Science, University of Rome Tor Vergata, Rome, Italy.
This work presents an innovative all-electrical platform for selective single-particle manipulation. The platform combines microfluidic impedance cytometry for label-free particle characterization and dielectrophoresis for contactless multi-way particle separation. The microfluidic chip has a straightforward coplanar electrode layout and no particle pre-focusing mechanism is required.
View Article and Find Full Text PDFBiosensors (Basel)
August 2024
2020 X-Lab, Shanghai Institute of Microsystem and Information Technology, Chinese Academy of Sciences, Shanghai 200050, China.
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