AI Article Synopsis
- Human cancer cell lines are crucial in drug discovery, but traditional 2D models fail to mimic the real tumor environment effectively.
- Advanced 3D cell culture models are being developed, providing better insights into cancer mechanisms and drug resistance.
- Recent research shows 3D models can enhance drug discovery, yet more work is needed to create models that fully replicate tumor complexity.
Article Abstract
Human cancer cell lines are an integral part of drug discovery practices. However, modeling the complexity of cancer utilizing these cell lines on standard plastic substrata, does not accurately represent the tumor microenvironment. Research into developing advanced tumor cell culture models in a three-dimensional (3D) architecture that more prescisely characterizes the disease state have been undertaken by a number of laboratories around the world. These 3D cell culture models are particularly beneficial for investigating mechanistic processes and drug resistance in tumor cells. In addition, a range of molecular mechanisms deconstructed by studying cancer cells in 3D models suggest that tumor cells cultured in two-dimensional monolayer conditions do not respond to cancer therapeutics/compounds in a similar manner. Recent studies have demonstrated the potential of utilizing 3D cell culture models in drug discovery programs; however, it is evident that further research is required for the development of more complex models that incorporate the majority of the cellular and physical properties of a tumor.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4085612 | PMC |
| http://dx.doi.org/10.3390/biology3020345 | DOI Listing |
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