AI Article Synopsis
- Eltrombopag is a medication used to treat low platelet counts (thrombocytopenia) and this study introduces a method for measuring its levels in human plasma.
- The method utilizes liquid chromatography-tandem mass spectrometry (LC-MS/MS) with a specific internal standard, extracting the analytes from only 50μL of plasma through a simple protein precipitation process.
- The assay demonstrated a strong linear response for eltrombopag concentrations, with high precision and accuracy, making it suitable for pharmacokinetic studies.
Article Abstract
Eltrombopag is a thrombopoietin receptor agonist, used in the treatment of thrombocytopenia. This paper describes a liquid chromatography-tandem mass spectrometry (LC-MS/MS) assay method for the determination of eltrombopag in human plasma samples using eltrombopag 13C4 as internal standard (IS). Analyte and the IS were extracted from 50μL of human plasma using protein precipitation technique with no drying, evaporation and reconstitution steps. The chromatographic separation was achieved on a C18 column by using a mixture of 10mM ammonium formate (pH3) and acetonitrile (10:90, v/v) as the mobile phase at a flow rate of 1.0mL/min. The linearity of the method was established in the concentration range 50.0-10007ng/mL with r(2)≥0.99. The intra-day and inter-day precision and accuracy results in four validation batches across five concentration levels were well within the acceptance limits. The proposed method was found to be applicable to pharmacokinetic studies.
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| http://dx.doi.org/10.1016/j.jpba.2014.04.028 | DOI Listing |
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