We report a method for generating Drosophila germline mutants effectively via injection of the complex of the purified Cas9 protein, tracrRNA, and gene-specific crRNAs, which may reduce delayed mutations because of the transient activity of the Cas9 protein, combined with the simple mutation detection in GO founders by the T7E1 assay.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4455777 | PMC |
| http://dx.doi.org/10.1534/g3.114.012179 | DOI Listing |
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