Background: The establishment of coral sperm repositories which retain good post-rewarming viability and fertility play a vital role in species conservation.
Objective: This study aimed at obtaining baseline information regarding the effects of cryoprotectant agents (CPAs) on gorgonian coral (Junceella juncea and J. fragilis) sperm sacs.
Methods: The adenosine triphosphate assay was used to determine the energy level of the gorgonian sperm sacs as an indicator of sperm viability after exposure to cryoprotectants.
Results: The 'no observed effect concentrations' (NOECs) of methanol, dimethyl sulfoxide (DMSO), polypropylene glycol (PG), ethylene glycol (EG) and glycerol for J. juncea sperm sacs were 3 M, 3 M, 1 M, 2 M and 1 M respectively after 20 min exposure; whilst the NOECs for J. fragilis oocytes were 2 M, 3 M, 1 M, 2 M and 2 M, respectively. Methanol and DMSO had the least impact. PG was the most toxic CPA after 10 min exposure. ATP content of J. juncea and J. fragilis sperm sacs did not differ significantly from the control with incubation times of 10-20 min with 2 M EG. However, ATP content dropped significantly after exposing sperm sacs to 2 M EG for 40 min with average values of 2.34 +/- 0.12 and 1.97 +/- 0.48 microg/ml respectively. ATP content for J. juncea and J. fragilis sperm sacs was significantly decreased to 1.79 +/- 0.31 and 2.40 +/- 0.36 microg/ml after 20 min incubation in 2 M PG when compared to the control with 2.98 +/- 0.16 and 4.14 +/- 0.42 microg/ml respectively. Normalized ATP content for sperm sacs of two different gorgonian coral after incubation in methanol, DMSO, PG, EG and glycerol showed that J. juncea sperm sacs were slightly less tolerant to CPAs compared to J. fragilis sperm sacs.
Conclusions: DMSO or methanol can be considered as efficient CPAs for gorgonian sperm sacs cryopreservation. The ATP luminescence assay provided sensitive and rapid quantification of mitochondrial activity in gorgonian coral sperm sacs. The study on the impact of CPA will contribute to the development of a cryopreservation protocol for coral sperm conservation.
Download full-text PDF |
Source |
|---|
Publication Analysis
Top Keywords
Similar Publications
Sexual conflict underlying external female genital mutilation in spiders: assessing whether females benefit from multiple matings.
Proc Biol Sci
January 2025
Faculty of Applied Information Technology, Nagasaki Institute of Applied Science, Nagasaki, Japan.
External female genital mutilation (EFGM) is a type of traumatic mating in which males damage female genitalia, resulting in the loss of female re-mating ability. This study examined whether sexual conflict underlies EFGM by examining the possible female reproductive costs from the decreased number of matings in spider, . The female typically receives sperm from a male twice during a mating bout.
View Article and Find Full Text PDFAlternative mating pattern in is associated with polyandry and male-biased sex ratio.
MicroPubl Biol
January 2025
Shimane University, Matsue, Shimane, Japan.
Although cephalopods are primarily polyandrous, genetic evidence revealed rare monogamy in . Here, we studied the sister species . We found that copulation began in early July, with egg spawning occurring in early August.
View Article and Find Full Text PDFUltrastructure of the ejaculatory duct of Terrobittacus implicatus (Mecoptera: Bittacidae).
Protoplasma
December 2024
Ultrastructural Pathology Diagnosis and Research Center, The First Affiliated Hospital of Henan University of Science and Technology, Luoyang, 471003, China.
The unique mating behavior of Bittacidae has been extensively studied, yet the mechanisms underlying internal sperm transport and temporary storage before mating remain enigmatic. Herein, we aim to elucidate these mechanisms by investigating the fine structure of the ejaculatory duct, which serves for sperm transport and temporary storage. The ultrastructure of the ejaculatory duct of Terrobittacus implicatus (Mecoptera: Bittacidae) was examined by light and transmission electron microscopy for the first time in this study.
View Article and Find Full Text PDFExploring swine oviduct anatomy through micro-computed tomography: a 3D modeling perspective.
Front Vet Sci
September 2024
Department of Biosciences and Technology for Food, Agriculture and Environment, University of Teramo, Teramo, Italy.
Article Synopsis
- The oviduct is super important for reproduction, helping with fertilization and where embryos start to develop.
- Researchers used special technology called MicroCT to carefully study two parts of the oviduct, finding differences in their shapes and sizes.
- The study showed that these structural differences might affect how the oviduct works, and it could help create a mini version of it for research.
Embryo sac cellularization defects lead to supernumerary egg cells and twin embryos in .
iScience
June 2024
CSIR-Institute of Himalayan Bioresource Technology, Palampur 176061, Himachal Pradesh, India.
lines with loss-of-function mutation in () gene showed seed sterility with embryo sac cellularization defects. Examination of tissue-cleared mature ovules revealed irregularly positioned nuclei/embryos within the embryo sacs. Egg cell-specific marker (DD45) expression analysis confirmed the presence of multiple egg cells in the mutant embryo sacs.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!