The effects of pre-treatment with surfactants on the electrocatalytic reaction of multi-copper oxidases were quantitatively evaluated using a well-structured carbon nanotube forest electrode. It was found that both the charge polarity of the head group and the aromatics in the tail part of the surfactants affect the efficiency of enzymatic electrocatalysis.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1039/c4cp00872c | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Influence of Ligand Complexity on the Spectroscopic Properties of Type 1 Copper Sites: A Theoretical Study.
J Comput Chem
January 2025
Department of Mechanical Engineering, Texas Tech University, Lubbock, Texas, USA.
Multi-copper oxidases (MCOs) are enzymes of significant interest in biotechnology due to their efficient catalysis of oxygen reduction to water, making them valuable in sustainable energy production and bio-electrochemical applications. This study employs time-dependent density functional theory (TDDFT) to investigate the electronic structure and spectroscopic properties of the Type 1 (T1) copper site in Azurin, which serves as a model for similar sites in MCOs. Four model complexes of varying complexity were derived from the T1 site, including 3 three-coordinate models and 1 four-coordinate model with axial methionine ligation, to explore the impact of molecular branches and axial coordination.
View Article and Find Full Text PDFPrecision Thermostability Predictions: Leveraging Machine Learning for Examining Laccases and Their Associated Genes.
Int J Mol Sci
December 2024
International Ph.D. Program in Biomedical Engineering, College of Biomedical Engineering, Taipei Medical University, Taipei 11031, Taiwan.
Laccases, multi-copper oxidases, play pivotal roles in the oxidation of a variety of substrates, impacting numerous biological functions and industrial processes. However, their industrial adoption has been limited by challenges in thermostability. This study employed advanced computational models, including random forest (RF) regressors and convolutional neural networks (CNNs), to predict and enhance the thermostability of laccases.
View Article and Find Full Text PDFOptimized production of laccase from Pseudomonas stutzeri and its biodegradation of lignin in biomass.
Folia Microbiol (Praha)
December 2024
National Institute for Biotechnology and Genetic Engineering (NIBGE), Jhang Road, P.O. Box 577, Faisalabad, Pakistan.
Laccases are multi-copper oxidases that play an important role in the biodegradation of phenolic compounds, lignin, dye, and wastes. Here, we report the screening of potential laccase-producing indigenous bacterial isolates and subsequent optimization of laccase production using crop residues as cheap supplementary energy sources. Among 16 bacterial isolates, seven were selected based on the appearance of reddish-brown bacterial colonies and guaiacol oxidation assay after 10 days of incubation at 37 °C.
View Article and Find Full Text PDFLaccase based per- and polyfluoroalkyl substances degradation: Status and future perspectives.
Water Res
March 2025
Section of Industrial Biotechnology, Department of Biological and Chemical Engineering, Aarhus University, Gustav Wieds Vej 10, Aarhus C, 8000, Denmark. Electronic address:
Per- and polyfluoroalkyl substances (PFAS) with stable carbon-fluorine bonds are used in a wide range of industrial and commercial applications. Due to their extreme environmental persistence, PFAS have the potential to bioaccumulate, cause adverse effects, and present challenges regarding remediation. Recently, microbial and enzymatic reactions for sustainable degradation of PFAS have gained attention from researchers, although biological decomposition of PFAS remains challenging.
View Article and Find Full Text PDFThermophilic lignin-based laccase nanozyme with CuN center for the detection of epinephrine and degradation of phenolic pollutants.
Int J Biol Macromol
December 2024
Beijing Key Laboratory of Lignocellulosic Chemistry, Beijing Forestry University, Beijing 100083, China; Engineering Research Center of Forestry Biomass Materials and Energy, Ministry of Education, Beijing Forestry University, Beijing 100083, China. Electronic address:
Natural laccases are a family of multi‑copper oxidases that can oxidize multiple phenol substrates and of great importance to contaminant remediation and biosensing. However, the construction of substitutes for the expensive and perishable laccase used in harsh conditions remains a great challenge. Here, we reported a novel strategy for the fabrication of copper-doped lignin-based laccase nanozymes (Cu-AL) through the coordination of aminated lignin and different copper sources.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!