Enzymatic parameters such as pH, temperature and substrate concentration were studied for the hydrolysis of 7-PADCA by penicillin G acylase. Optimum pH and temperature were 8.0 and 50 degrees C, respectively. Km value of soluble and immobilized enzyme for 7-PADCA was 2.3 x 10(-5) M and 7.5 x 10(-5) M, respectively. At 7-PADCA concentration of 5% and an IME: 7-PADCA ratio of 1:2.5, the hydrolysis was complete in 110 min.
Download full-text PDF |
Source |
|---|
Publication Analysis
Top Keywords
Similar Publications
Chlorogenic Acid: A Promising Strategy for Milk Preservation by Inhibiting Growth and Biofilm Formation.
Foods
December 2024
College of Food Science &Technology, Yunnan Agricultural University, Kunming 650201, China.
Chlorogenic acid (CGA), a polyhydroxy phenolic acid, has been extensively studied for its antimicrobial properties. () threatens food safety by forming biofilms. This study aimed to investigate the mechanism of CGA against and its biofilm.
View Article and Find Full Text PDFStudy of the poly (butylene adipate-co-terephthalate)/poly (vinyl alcohol) coated recycled FeO magnetic particle carriers for immobilization penicillin G acylase.
J Biomater Sci Polym Ed
November 2024
College of Materials Science and Engineering, Lanzhou University of Technology, Lanzhou, China.
Article Synopsis
- * The specific enzyme (PGA) was successfully immobilized through a chemical reaction, and optimal conditions for this process were identified with a 93.71% enzyme activity recovery and significant loading capacity.
- * The immobilized PGA demonstrated better stability and efficiency, retaining 58% of its initial activity after 11 uses, suggesting its potential for practical applications in various fields.
Strategies to enhance the hydrolytic activity of Escherichia coli BL21 penicillin G acylase based on heterologous expression and targeted mutagenesis.
Colloids Surf B Biointerfaces
February 2025
School of Life Sciences, Ningxia University, Yinchuan, Ningxia 750021, China; School of Food Science and Engineering, Ningxia University, Yinchuan, Ningxia 750021, China; Ningxia Key Laboratory for Food Microbial-Applications Technology and Safety Control, Ningxia University, Yinchuan, Ningxia 750021, China. Electronic address:
Penicillin G acylase (PGA) serves as a critical biocatalyst for the hydrolysis of penicillin G, yielding 6-aminopenicillanic acid, a vital precursor for β-lactam semi-synthetic antibiotics. The catalytic efficiency of PGA, however, remains suboptimal in native Escherichia coli strains. To improve this, E.
View Article and Find Full Text PDFAn Overview of Microorganisms Immobilized in a Gel Structure for the Production of Precursors, Antibiotics, and Valuable Products.
Gels
October 2024
JSC "Scientific Center of Anti-Infective Drugs", Almaty 050000, Kazakhstan.
Using free microorganisms for industrial processes has some limitations, such as the extensive consumption of substrates for growth, significant sensitivity to the microenvironment, and the necessity of separation from the product and, therefore, the cyclic process. It is widely acknowledged that confining or immobilizing cells in a matrix or support structure enhances enzyme stability, facilitates recycling, enhances rheological resilience, lowers bioprocess costs, and serves as a fundamental prerequisite for large-scale applications. This report summarizes the various cell immobilization methods, including several synthetic (polyvinylalcohol, polyethylenimine, polyacrylates, and Eudragit) and natural (gelatin, chitosan, alginate, cellulose, agar-agar, carboxymethylcellulose, and other polysaccharides) polymeric materials in the form of thin films, hydrogels, and cryogels.
View Article and Find Full Text PDFIn Vivo Cross-Linking Sheds Light on the Salmonella Divisome in Which PBP3 and PBP3 Compete for Occupancy.
Mol Microbiol
November 2024
Laboratory of Intracellular Bacterial Pathogens, National Centre for Biotechnology (CNB-CSIC), Madrid, Spain.
Bacterial cell division is orchestrated by proteins that assemble in dynamic complexes collectively known as the divisome. Essential monofunctional enzymes with glycosyltransferase or transpeptidase (TPase) activities, FtsW and FtsI respectively, engage in the synthesis of septal peptidoglycan (sPG). Enigmatically, Salmonella has two TPases that can promote cell division independently: FtsI (PBP3) and the pathogen-specific paralogue PBP3.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!