AI Article Synopsis
- 4-Hydroxy-2-nonenal (4HNE) is an important chemical formed during lipid breakdown and is linked to diseases like rheumatoid arthritis, Parkinson's, and cancer.
- A new method using High-Performance Liquid Chromatography (HPLC) with fluorescence detection was created to accurately measure 4HNE levels in human serum.
- This method includes extracting 4HNE at very low temperatures and tagging it for fluorescence, achieving a low detection limit of 0.06 µm, and has been effectively tested on serum samples from rheumatoid arthritis patients.
Article Abstract
4-Hydroxy-2-nonenal (4HNE) is a major aldehyde generated during lipid peroxidation. The clinical monitoring of 4HNE in biological fluids could be useful for the early diagnosis of several diseases involving lipid peroxidation, such as rheumatoid arthritis, Parkinson's disease and cancer. In this study, an HPLC with fluorescence detection method was developed for the determination of 4HNE in human serum. The proposed method involves the extraction of 4HNE from human serum by subzero temperature extraction and fluorescent labeling of 4HNE with 4-(N,N-dimethylaminosulfonyl)-7-hydrazino-2,1,3-benzoxadiazole. The lower detection limit (signal-to-noise ratio=3) of the method was 0.06 µm in serum. The proposed method was successfully applied to the measurement of 4HNE in sera obtained from patients with rheumatoid arthritis.
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| http://dx.doi.org/10.1002/bmc.3204 | DOI Listing |
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