The cleft palate presented by transforming growth factor-β3 (Tgf-β3) null mutant mice is caused by altered palatal shelf adhesion, cell proliferation, epithelial-to-mesenchymal transformation and cell death. The expression of epidermal growth factor (EGF), transforming growth factor-β1 (Tgf-β1) and muscle segment homeobox-1 (Msx-1) is modified in the palates of these knockout mice, and the cell proliferation defect is caused by the change in EGF expression. In this study, we aimed to determine whether this change in EGF expression has any effect on the other mechanisms altered in Tgf-β3 knockout mouse palates. We tested the effect of inhibiting EGF activity in vitro in the knockout palates via the addition of Tyrphostin AG 1478. We also investigated possible interactions between EGF, Tgf-β1 and Msx-1 in Tgf-β3 null mouse palate cultures. The results show that the inhibition of EGF activity in Tgf-β3 null mouse palate cultures improves palatal shelf adhesion and fusion, with a particular effect on cell death, and restores the normal distribution pattern of Msx-1 in the palatal mesenchyme. Inhibition of TGF-β1 does not affect either EGF or Msx-1 expression.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1159/000362227 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Transcriptional factor ISL1 regulates palate development by tuning the SHH cascade.
FEBS J
December 2024
Zhejiang Key Laboratory of Organ Development and Regeneration, Institute of Developmental and Regenerative Biology, College of Life and Environmental Sciences, Hangzhou Normal University, China.
Cleft palate is one of the most common birth defects in humans, and palate morphogenesis depends on epithelial-mesenchymal interaction. In this study, we report that ablation of Isl1 in the epithelium leads to complete cleft palate. A significant reduction in mesenchymal cell proliferation was detected in the Isl1 mutant palates, but there was no significant difference in apoptosis between wild-type and mutant embryos.
View Article and Find Full Text PDFAssembly of Custom Implant and Ready-Made Distractor for Precise Maxillary Movement Vector Control in Cleft Patient With Midfacial Hypoplasia.
J Craniofac Surg
November 2024
Department of Oral and Maxillofacial Surgery, Chung-Ang University Hospital, Seoul, Korea.
This study presents a novel approach for maxillary advancement in a patient with cleft lip and palate, utilizing the assembly of a custom titanium implant and a ready-made distractor. The patient exhibited significant maxillary hypoplasia and had concerns regarding the possible deterioration of hypernasality after conventional surgical methods. Distraction osteogenesis was initiated to address these challenges.
View Article and Find Full Text PDFCleft Palate Induced by Augmented Fibroblast Growth Factor-9 Signaling in Cranial Neural Crest Cells in Mice.
Stem Cells Dev
October 2024
Fujian Key Laboratory of Oral Diseases & Fujian Provincial Engineering Research Center of Oral Biomaterial & Stomatological Key Lab of Fujian College and University, School and Hospital of Stomatology, Fujian Medical University, Fuzhou, P.R. China.
Although enhanced fibroblast growth factor (FGF) signaling has been demonstrated to be crucial in many cases of syndromic cleft palate caused by tongue malposition in humans, animal models that recapitulate this phenotype are limited, and the precise mechanisms remain elusive. Mutations in with the effect of either loss- or gain-of-function effects have been identified to be associated with cleft palate in humans. Here, we generated a mouse model with a transgenic allele specifically activated in cranial neural crest cells, aiming to elucidate the gain-of-function effects of in palatogenesis.
View Article and Find Full Text PDFComputational modeling of maxillary canine orthodontic movement.
Heliyon
July 2024
The Goldschleger School of Dental Medicine, Faculty of Medical and Health Sciences, Tel Aviv University, Tel Aviv, 6997801, Israel.
Objectives: The current study aims to explore the stress distribution along the roots of palatally positioned maxillary canines during orthodontic movement using a novel computational spring model.
Methods: An experimental analysis based on the spring-model was utilized to calculate Orthodontic Tooth Movement (OTM) and the resulting stresses. Two sets of experiments were conducted: the first set compared stresses on a canine resulting from a single force and a force-couple, while the second set simulated canines' traction during instantaneous movement with varying original tooth angulations using different off-the-shelf orthodontic coils.
Development of sensory tools for green rooibos (Aspalathus linearis (Burm.f.) R.Dahlgren) and changes in quality attributes during shelf-life storage.
J Sci Food Agric
September 2024
Plant Bioactives Group, Post-Harvest and Agro-Processing Technologies, Agricultural Research Council (Infruitec-Nietvoorbij), Stellenbosch, South Africa.
Background: Green rooibos (Aspalathus linearis (Burm.f.) R.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!