AI Article Synopsis
- The study investigated the role of two genes, PmRab7 and PmIAP, in shrimp's immune response after vaccination and infection with white spot syndrome virus (WSSV).
- Shrimps were vaccinated with WSSV-coated feed and then were injected with dsRNA to silence either the PmRab7 or PmIAP genes before exposure to the virus.
- The results showed that silencing PmRab7 led to down-regulation of immune-related genes, while silencing PmIAP affected apoptosis-related genes, highlighting their significance in the shrimp's response to viral infection.
Article Abstract
In the present study RNA interference was used to elucidate the connection between two endogenous genes [Penaeus monodon Rab7 (PmRab7) or P. monodon inhibitor of apoptosis (PmIAP)], and selected immune/apoptosis-related genes in orally 'vaccinated' shrimp after white spot syndrome virus (WSSV) infection. P. monodon were vaccinated by feeding them with formalin inactivated WSSV-coated feed. Thereafter, PmRab7 or PmIAP genes were silenced by injecting the shrimps with their respective dsRNA. The resulting groups of shrimps, Rab7 and IAP, were orally infected with WSSV and the expression of three immune-relevant genes in Rab7 group and five apoptosis-related genes in IAP group was evaluated. In the Rab7 group, PmToll, PmPPAE 2 and Pm penaeidin genes were down-regulated. The IAP-silenced shrimps were characterized by down-regulation of Pm caspase, PmERp57, Pm14-3-3 ε, Pm ald, and up-regulation of PmSTAT. Thus, silencing of PmRab7/PmIAP has provided important clues on their relationship with selected immune/apoptosis genes in orally vaccinated P. monodon during WSSV infection.
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| http://dx.doi.org/10.1016/j.margen.2014.05.002 | DOI Listing |
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