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The ability of inner-cell-mass cells to self-renew as embryonic stem cells is acquired following epiblast specification. | LitMetric

The ability of inner-cell-mass cells to self-renew as embryonic stem cells is acquired following epiblast specification.

Nat Cell Biol

1] Wellcome Trust - Medical Research Council Cambridge Stem Cell Institute, University of Cambridge, Tennis Court Road Cambridge CB2 1QR, UK [2] Department of Physiology, Development and Neuroscience, University of Cambridge, Cambridge CB2 3EG, UK.

Published: June 2014

AI Article Synopsis

Article Abstract

The precise relationship of embryonic stem cells (ESCs) to cells in the mouse embryo remains controversial. We present transcriptional and functional data to identify the embryonic counterpart of ESCs. Marker profiling shows that ESCs are distinct from early inner cell mass (ICM) and closely resemble pre-implantation epiblast. A characteristic feature of mouse ESCs is propagation without ERK signalling. Single-cell culture reveals that cell-autonomous capacity to thrive when the ERK pathway is inhibited arises late during blastocyst development and is lost after implantation. The frequency of deriving clonal ESC lines suggests that all E4.5 epiblast cells can become ESCs. We further show that ICM cells from early blastocysts can progress to ERK independence if provided with a specific laminin substrate. These findings suggest that formation of the epiblast coincides with competence for ERK-independent self-renewal in vitro and consequent propagation as ESC lines.

Download full-text PDF

Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4878656PMC
http://dx.doi.org/10.1038/ncb2965DOI Listing

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