We determined nucleotide and deduced amino acid sequences of the rotavirus gene encoding viral protein 6 from 3 fecal samples collected from piglets with diarrhea in Brazil, 2012. The analyses showed that the porcine rotavirus strains in Brazil are closely related to the novel species H rotavirus.
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http://dx.doi.org/10.3201/eid2006.130776 | DOI Listing |
Microorganisms
January 2025
Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming 650500, China.
Rotavirus A (RVA) is the primary enteric pathogen of humans and many other species. However, RVA interspecies transmission remains poorly understood. In this study, we conducted a comprehensive screening and genotyping analysis of RVA in 1706 wild animal samples collected from various regions within Yunnan Province, China.
View Article and Find Full Text PDFJ Gen Virol
January 2025
National Key Laboratory of Intelligent Tracking and Forecasting for Infectious Diseases (NITFID), NHC Key Laboratory for Medical Virology and Viral Diseases, National Institute for Viral Disease Control and Prevention, Beijing 100052, PR China.
Species A rotaviruses (RVs), which belong to the family and contain a genome of 11 segmented dsRNA segments, are a leading cause of severe acute gastroenteritis in infants and children younger than 5 years of age. We previously developed a strategy to recover rotavirus vaccine strain LLR from 11 cloned plasmids. Here, we report an improved reverse genetics system for LLR by combining two or three transcriptional cassettes in a single plasmid, which substantially enhances rescue efficiency from 66.
View Article and Find Full Text PDFPathogens
November 2024
College of Animal Science and Technology, Guangxi University, Nanning 530005, China.
Porcine astrovirus (PoAstV), porcine sapovirus (PoSaV), porcine norovirus (PoNoV), and porcine rotavirus A (PoRVA) are newly discovered important porcine diarrhea viruses with a wide range of hosts and zoonotic potential, and their co-infections are often found in pig herds. In this study, the specific primers and probes were designed targeting the ORF1 gene of PoAstV, PoSaV, and PoNoV, and the VP6 gene of PoRVA. The recombinant standard plasmids were constructed, the reaction conditions (concentration of primers and probes, annealing temperature, and reaction cycle) were optimized, and the specificity, sensitivity, and reproducibility were analyzed to establish a quadruplex real-time quantitative RT-PCR (RT-qPCR) assay for the detection of these four diarrheal viruses.
View Article and Find Full Text PDFTalanta
April 2025
College of Animal Science and Technology, Guangxi Key Laboratory of Animal Breeding, Disease Control and Prevention, Guangxi University, Nanning, 530004, China. Electronic address:
Porcine epidemic diarrhea virus (PEDV) and porcine rotavirus (PoRV) are the two main pathogens causing porcine diarrhea, which are characterized by high morbidity and mortality. Most of the diagnostic methods available are limited to the laboratory or fail to highlight their advantages in terms of target species, detection time, sensitivity, and stability. To meet the demand for rapid on-site diagnosis of PEDV and PoRV co-infection, a surface-enhanced Raman scattering (SERS) immunochromatographic sensor based on gold magnetic nanoparticles (MNPs) was developed.
View Article and Find Full Text PDFVirol Sin
December 2024
National Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, China; The Cooperative Innovation Center for Sustainable Pig Production, Wuhan 430070, China; Hubei Hongshan Laboratory, Wuhan 430070, China. Electronic address:
Rotavirus infections cause severe gastroenteritis and dehydration in young children and animals worldwide, leading to high rates of morbidity and mortality, predominantly in low- and middle-income countries. In the past decade, substantial progress has been made in the development and implementation of rotavirus vaccines, which have been essential in alleviating the global burden of this disease, not only in human being but also in livestock species like calves and piglets, where these infections can cause significant economic losses. By synthesizing the latest research and real-world evidence, this review article is designated to provide deep insights into the current state of rotavirus vaccine technology and its global implementation as well as the application of rotavirus vaccines in veterinary settings and their importance in controlling zoonotic transmission and maintaining food security.
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