The potassium-dependent alkaliphilic Bacillus sp. strain TS-2 was isolated from the mashed extract of a jumping spider, and its draft genome sequence was obtained. Comparative genomic analysis with a previously sequenced sodium-dependent alkaliphilic Bacillus species may reveal potassium-dependent alkaline adaptation mechanisms.
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http://dx.doi.org/10.1128/genomeA.00458-14 | DOI Listing |
Carbohydr Res
December 2024
Quantitative Biology Lab, Department of Integrative Biology, School of Bio Sciences and Technology, Vellore Institute of Technology (VIT Deemed to Be University), Vellore, Tamil Nadu, India. Electronic address:
Pectate lyases, known for their alkaliphilic nature, are ideal for industrial applications that require specific pH conditions, particularly in industries such as textiles and pulp extraction. These enzymes, primarily from the polysaccharide lyase family 1 (PL1) of different microbial sources, play a vital role in polysaccharide degradation. Given the potent pectinolytic activity of Bacillus pectate lyases, targeting these enzymes is crucial for identifying the most effective candidates.
View Article and Find Full Text PDFMicrob Cell Fact
November 2024
State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing, 100101, China.
Curr Microbiol
October 2024
Marine Biotechnology Division, National Institute of Ocean Technology (NIOT), Ministry of Earth Sciences (MoES), Government of India, Pallikaranai, Chennai, 600100, India.
Arabian Sea is a highly productive Ocean owing to deep upwelling with reports on phosphorus cycling in ocean sediments. In this study, microbes from sea mounts of the Arabian Sea at varying depths (400 m, 900 m) were screened to isolate and characterize phosphate-solubilizing bacteria (PSB) with plant growth-promoting properties. Out of the seven morphologically different PSBs, two bacterial strains with maximum phosphate solubilization index were identified as Priestia megaterium (H1) and Bacillus velezensis (H2) based on biochemical and molecular characteristics.
View Article and Find Full Text PDFFront Microbiol
September 2024
Howard Hughes Medical Institute, Yale University, New Haven, CT, United States.
An improved approach was developed for the genetic manipulation of the Gram-positive extremophile (formerly called ). We describe an allelic replacement method originally developed for that allows the deletion, mutation, or insertion of genes without leaving markers or other genetic scars. In addition, a protocol for rapid plasmid methylation and transformation is presented.
View Article and Find Full Text PDFInt J Microbiol
July 2024
Laboratory of Molecular Biotechnology Institute of Ecology and Genetics of Microorganisms Ural Branch Russian Academy of Sciences, Golev Str., 13, Perm 614081, Russia.
The goal of the study is to evaluate metabolic and morphological changes of the facultative alkaliphile 5-DB and the weakly alkali-resistant ATCC 6633 in a wide pH range and at different NaCl concentrations. The alkaliphile 5-DB is shown to have a broader general resistance to adverse factors (wide pH range, 50 g/L NaCl) than a weakly alkali-tolerant strain of the same genus. This alkaliphile is also shown to have a significantly greater resistance not only to high pH but also to low pH in comparison with ATCC 6633.
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