AI Article Synopsis
Article Abstract
This study aims to assess whether soluble HLA-G (sHLA-G) is involved in apoptosis of decidual natural killer (dNK) cells following Toxoplasma gondii infection. dNK cells or NK-92 cells were infected with T. gondii and co-cultured with trophoblast cells or BeWo cells. Infected co-cultured cells were treated without or with sHLA-G neutralizing antibody. Uninfected co-cultured cells were used as controls. Apoptosis of dNK cells were analyzed by flow cytometry and confocal microscope. Real-time PCR and Western blot were used to determine caspase 3 and caspase 8 expression. sHLA-G in supernatant were measured by enzyme-linked immunosorbent assay (ELISA). In infection groups, sHLA-G was increased, while dNK apoptosis proteins caspase 3 and caspase 8 were up-regulated, but significantly decreased in the presence of sHLA-G neutralizing antibody compared to controls. Under the situation of T. gondii-infected dNK cells co-cultured with trophoblast cells, the up-regulation of sHLA-G could induce dNK cells apoptosis which ultimately may contribute to the abnormal pregnancy outcomes with T. gondii infection.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1007/s10753-014-9900-7 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Immune dysregulation of decidual NK cells mediated by GRIM19 downregulation contributes to the occurrence of recurrent pregnancy loss.
Mol Cell Biochem
December 2024
Department of Obstetrics and Gynecology, Center for Reproductive Medicine, Qilu Hospital of Shandong University, No.44 Wenhua Xi Road, Jinan, 250012, Shandong, China.
In patients with recurrent pregnancy loss (RPL), excessive activation of decidual natural killer (dNK) cells has been widely observed, yet the precise underlying mechanisms remain to be elucidated. We collected decidual specimens from RPL patients and controls to assess GRIM19 expression, activation phenotype, cytotoxic function, inflammatory cytokine secretion, and mitochondrial homeostasis in dNK cells. Furthermore, we established a GRIM19-knockout NK-92MI cell line and a GRIM19 ± C57BL/6J mouse model to investigate the relationship between GRIM19 downregulation and dNK immune dysregulation, ultimately contributing to pregnancy loss.
View Article and Find Full Text PDFUnderstanding the heterogeneity of natural killer cells at the maternal-fetal interface: implications for pregnancy health and disease.
Mol Hum Reprod
November 2024
Shenzhen Key Laboratory of Reproductive Immunology for Peri-Implantation, Shenzhen Zhongshan Institute for Reproductive Medicine and Genetics, Shenzhen Zhongshan Obstetrics & Gynecology Hospital (formerly Shenzhen Zhongshan Urology Hospital), Shenzhen, China.
Natural killer (NK) cells are the most abundant leukocytes located at the maternal-fetal interface; they respond to pregnancy-related hormones and play a pivotal role in maintaining the homeostatic micro-environment during pregnancy. However, due to the high heterogeneity of NK cell subsets, their categorization has been controversial. Here, we review previous studies on uterine NK cell subsets, including the classic categorization based on surface markers, functional molecules, and developmental stages, as well as single-cell RNA sequencing-based clustering approaches.
View Article and Find Full Text PDFAbnormal miR-122-5p expression in decidual NK cells and its impact on trophoblast behavior: insights into unexplained recurrent pregnancy loss.
Int J Med Sci
November 2024
Center of Translational Medicine, Key Laboratory of Birth Defects and Related Diseases of Women and Children of Ministry of Education, West China Second University Hospital, Sichuan University, Chengdu, 610041, China.
Article Synopsis
- Early pregnancy features high levels of natural killer (NK) cells at the maternal-fetal interface, which are crucial for fetal development and remodeling uterine arteries.
- Aberrant activity of decidual NK (dNK) cells is linked to recurrent pregnancy loss (RPL), but the exact molecular mechanisms behind their function need more research.
- This study highlights that downregulation of miR-122-5p in dNK cells from RPL patients affects their function, suggesting that miR-122-5p may play a role in maintaining immune tolerance during pregnancy.
TNFSF14 natural killer cells prevent spontaneous abortion by restricting leucine-mediated decidual stromal cell senescence.
EMBO J
November 2024
Department of Reproductive Immunology, The International Peace Maternity and Child Health Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, 200030, People's Republic of China.
Article Synopsis
- The endometrium transforms into a structure called decidua during pregnancy preparation, with senescent decidual stromal cells (DSCs) increasing but poorly understood mechanisms.
- Researchers used single-cell RNA transcriptomics to discover that higher levels of the BCAA transporter SLC3A2 accompany cell senescence in DSCs during decidualization.
- A high leucine diet contributes to increased DSC senescence and embryo loss in mice, while dNK cells can counteract this senescence through the TNFSF14/TNFRSF14 pathway, highlighting potential for therapeutic interventions in preventing pregnancy loss.
Gene Regulatory Network Analysis of Decidual Stromal Cells and Natural Killer Cells.
Reprod Sci
October 2024
Turku Bioscience Centre, University of Turku, Åbo Akademi University, Turku, Finland.
Article Synopsis
- Human reproductive success hinges on the proper development of the uterine endometrium, which is essential for implantation and pregnancy, involving key cell types: decidual stromal cells (dS) and decidual natural killer (dNK) cells.
- The study used single-cell transcriptomics from early pregnancy to uncover new transcription factors affecting these cell types, revealing factors like DDIT3 and BRF2 in dS cells that help manage oxidative stress and factors like IRX3 and RELB in dNK cells that promote immune tolerance.
- The research also linked these findings to pregnancy disorders, showing that specific transcription factors are significantly associated with gene expression changes observed in conditions like recurrent pregnancy loss and preeclampsia.*
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!