Materials are submitted on electron-microscopic studying of alive and inactivated Coxiella in their interaction with murine cells during 28 days observations. The most variability of Coxiella morphologic types is shown to be found in spleen. The structure of endospora type is described. Several types of phagosomes are revealed and described.
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Sci Adv
January 2025
Division of Chemistry and Chemical Engineering, California Institute of Technology, Pasadena, CA, USA.
Many bacteria live in polymeric fluids, such as mucus, environmental polysaccharides, and extracellular polymers in biofilms. However, laboratory studies typically focus on cells in polymer-free fluids. Here, we show that interactions with polymers shape a fundamental feature of bacterial life-how they proliferate in space in multicellular colonies.
View Article and Find Full Text PDFJ Exp Bot
January 2025
Plant-Fusarium Interactions Research Team, School of BioSciences, University of Melbourne, Parkville, Australia.
Jasmonic acid (JA), ethylene (ET) and salicylic acid (SA) are the three major phytohormones coordinating plant defense responses, and all three are implicated in the defense against the fungal pathogen Fusarium oxysporum. However, their distinct modes of action and possible interactions remain unknown, in part because all spatial information on their activity is lacking. Here, we set out to probe this spatial aspect of plant immunity by using live-microscopy with newly developed fluorescence-based transcriptional reporter lines.
View Article and Find Full Text PDFAnnu Rev Psychol
January 2025
Department of Psychology, Northeastern University, Boston, Massachusetts, USA.
People often want to know what their interaction partners are thinking. How accurate are they, what information do they use, what predicts how accurate they will be, and does accuracy matter? We organize our review of thought-feeling accuracy, defined as the accuracy of individuals' judgments about the content of another person's thoughts and feelings in live interaction, around these questions. At the same time, we argue that often people are especially interested in what others are thinking about them, such that research on the accuracy of individuals' metaperceptions regarding others' views of them is highly relevant to understanding thought-feeling accuracy more broadly construed.
View Article and Find Full Text PDFMethods Mol Biol
January 2025
Department of Internal Medicine II, University Hospital Frankfurt, Goethe University Frankfurt, Frankfurt am Main, Germany.
Spheroid culture systems have been extensively used to model the three-dimensional (3D) behavior of cells in vitro. Traditionally, spheroids consist of a single cell type, limiting their ability to fully recapitulate the complex inter-cellular interactions observed in vivo. Here we describe a protocol for generating cocultured spheroids composed of two distinct cell types, embedded within a 3D extracellular matrix (ECM) to better study cellular interactions.
View Article and Find Full Text PDFEcohealth
January 2025
Department of Pathobiology, University of Illinois College of Veterinary Medicine, Urbana, IL, USA.
SARS-CoV-2 has been found in multiple species, including cervids such as wild white-tailed deer (WTD), in multiple regions in the United States, including Illinois. The virus has been shown to transmit among WTD, and across species in both directions (deer-to-humans and humans-to-deer). Cross-species transmission requires infectious contact between WTD and humans, the form and frequency of which is poorly understood.
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