The Warthin-Starry stain have been used for coloring a different microorganism like spirochetas, Donovan bodies and Campylobacter and also melanin granules demonstration in soft tissues tumors. We started using the stain, as we know it, in order to stain the Histolytica amebas. We observed parasite stained black and brown as well as a good differentiation between endoplasm and ectoplasm where granular appearance, bacterial remnants and red cells were apparent. The stain was also usefull in differentiating amebas from histiocytes. We advice its use in amebiasis since is inexpensive and easy to do.
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Diagn Microbiol Infect Dis
January 2025
Division of Infectious Diseases, Naval Medical Center San Diego, 34800 Bob Wilson Drive, San Diego, CA, 92134, USA; Department of Medicine, Uniformed Services University, 4301 Jones Bridge Road, Bethesda, MD 20814, USA.
Entamoeba histolytica infections range from asymptomatic intestinal amebiasis to more severe syndromes like invasive colitis or hepatic abscess. While diagnostic tests available for assessing these infections have evolved, the optimal use of newer diagnostics like enteric multiplex Polymerase Chain Reaction (PCR) panels has not been fully established. Here we describe the case of a 34-year-old male with an amebic liver abscess (ALA) that was diagnosed via multiplex Gastrointestinal (GI) PCR panel on formed stool and subsequently confirmed by testing liver abscess fluid (off-label) on the same multiplex GI PCR panel leading to rapid diagnosis and targeted treatment modification with excellent outcome.
View Article and Find Full Text PDFTurkiye Parazitol Derg
January 2025
Erzincan Binali Yıldırım University Faculty of Medicine, Department of Medical Microbiology, Erzincan, Türkiye.
Objective: To determine the prevalence of amoebiasis, which has been neglected in recent years according to the World Health Organization, in ulcerative colitis patients and investigate the relationship between amoebiasis and ulcerative colitis.
Methods: The study included 150 individuals, including 100 ulcerative colitis patients and 50 healthy individuals without gastrointestinal complaints. The samples collected were first analyzed macroscopically and then using native-Lugol, trichrome staining, and enzyme-linked immunosorbent assay (ELISA).
Turkiye Parazitol Derg
January 2025
Sağlık Bilimleri Üniversitesi, Dr. Behçet Uz Çocuk Hastalıkları ve Cerrahisi Eğitim Araştırma Hastanesi, Mikrobiyoloji Laboratuvarı, İzmir, Türkiye.
Objective: In the study, various complaints such as gastrointestinal complaints such as diarrhea, constipation, abdominal pain, nausea and vomiting, weight loss, night urination, itching around the anus, malnutrition, malabsorption, anemia, intelligence and developmental delay were reported to University of Health Sciences Türkiye, Dr. Behçet Uz Pediatric Diseases and Surgery Training and Researhc Hospital, Microbiology Laboratory between 2017 and 2022. It was aimed to determine the frequency and distribution of intestinal parasites in patients presenting with clinical symptoms.
View Article and Find Full Text PDFAnal Chim Acta
February 2025
Department of Medical Microbiology and Parasitology, School of Basic Medical Sciences, Fudan University, Shanghai, 200032, PR China; Shanghai Institute of Infectious Disease and Biosecurity, Fudan University, Shanghai, 200032, PR China. Electronic address:
Background: Entamoeba histolytica is a parasite that could cause severe amebiasis, an extremely contagious parasitic disease with critical clinical symptoms. Timely diagnosis and treatment of E. histolytica are crucial for preventing complications and fatalities.
View Article and Find Full Text PDFFront Parasitol
March 2024
Departamento de Infectómica y Patogénesis Molecular, Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional [CINVESTAV-Instituto Politécnico Nacional (IPN)], Mexico City, Mexico.
The retromer is a highly conserved eukaryotic complex formed by the cargo selective complex (CSC) and the sorting nexin (SNX) dimer subcomplexes. Its function is protein recycling and recovery from the endosomes to conduct the target molecules to the trans-Golgi network or the plasma membrane. The protozoan responsible for human amoebiasis, , exhibits an active membrane movement and voracious phagocytosis, events in which the retromer may be fully involved.
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