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LC-MS analysis of polyclonal IgGs using IdeS enzymatic proteolysis for oxidation monitoring. | LitMetric

LC-MS analysis of polyclonal IgGs using IdeS enzymatic proteolysis for oxidation monitoring.

J Chromatogr B Analyt Technol Biomed Life Sci

Analytical Department, LFB Biotechnologies, 3 Avenue des Tropiques, 91958 Courtaboeuf (Les Ulis), France. Electronic address:

Published: June 2014

AI Article Synopsis

Article Abstract

Susceptibility of IgGs to oxidation is a significant issue for intravenous immunoglobulin preparations (IVIG) in liquid solution and raises both safety and efficacy concerns. Here we present an optimized chromatography method coupled to mass spectrometry (MS) to determine the oxidation of Fc/2 fragments derived from polyclonal IgGs after IdeS treatment. Separation of the four IgG subclasses was achieved using a diphenyl column and UV/MS detections were used for quantification and characterization. Several oxidized Fc/2 fragments generated by stress conditions were resolved and oxidized methionines were identified. This procedure can be used to monitor the oxidative status of IVIG preparations during formulation or stability studies.

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.jchromb.2014.04.053DOI Listing

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