AI Article Synopsis

  • The text discusses the challenges of developing accurate models for testing new therapies in renal cell carcinoma (RCC), particularly those that mimic the disease's progression in patients.
  • It outlines a protocol that utilizes a highly metastatic mouse tumor cell line in a syngeneic model for orthotopic tumor implantation, incorporating methods to prevent early spread and leakage.
  • The protocol also includes procedures for kidney tumor excision and subsequent care, as well as techniques for monitoring and assessing disease progression, aiming to enhance the predictive value of therapeutic testing in RCC.

Article Abstract

One of the key challenges to improved testing of new experimental therapeutics in renal cell carcinoma (RCC) is the development of models that faithfully recapitulate early- and late-stage metastatic disease progression. Typical tumor implantation models utilize ectopic or orthotopic primary tumor implantation, but few include systemic spontaneous metastatic disease that mimics the clinical setting. This protocol describes the key steps to develop RCC disease progression stages similar to patients. First, it uses a highly metastatic mouse tumor cell line in a syngeneic model to show orthotopic tumor cell implantation. Methods include superficial and internal implantation into the sub-capsular space with cells combined with matrigel to prevent leakage and early spread. Next it describes the procedures for excision of tumor-bearing kidney (nephrectomy), with critical pre- and post- surgical mouse care. Finally, it outlines the steps necessary to monitor and assess micro-and macro-metastatic disease progression, including bioluminescent imaging as well provides a detailed visual necropsy guide to score systemic disease distribution. The goal of this protocol description is to facilitate the widespread use of clinically relevant metastatic RCC models to improve the predictive value of future therapeutic testing. 

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Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4183115PMC
http://dx.doi.org/10.3791/51485DOI Listing

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