Based on in vitro assays, we performed a High Throughput Screening (HTS) to identify kinase inhibitors among 10,000 small chemical compounds. In this didactic paper, we describe step-by-step the approach to validate the hits as well as the major pitfalls encountered in the development of active molecules. We propose a decision tree that could be adapted to most in vitro HTS.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4009756 | PMC |
| http://dx.doi.org/10.3390/biology3010167 | DOI Listing |
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