AI Article Synopsis
- * Abnormal levels of miRNAs are linked to various diseases, particularly cancer, which has sparked interest in developing miRNA-based therapies.
- * Researchers have developed methods to create miRNA inhibitors that can provide prolonged suppression of miRNA activity, facilitating long-term studies in cell cultures, cancer models, and living organisms.
Article Abstract
MicroRNAs (miRNAs) are a class of small RNAs (18-22 nt) that post transcriptionally regulate gene expression by binding to complementary sequences on target mRNAs, resulting in translational repression or target degradation and gene silencing. As aberrant expression of miRNAs is implicated in important diseases including cancer miRNA-based therapies are under intensive investigation. We optimized strategies to stably or conditionally generate miRNA inhibitors for a continuous block of miRNA activity that allows for probing miRNA function in long-term cell culture experiments, cancer xenografts, 3D tissue models and for in vivo studies with transgenic organisms.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3960870 | PMC |
| http://dx.doi.org/10.3390/biology2030861 | DOI Listing |
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