Taking Yangmai 18, widely cultivated in south of Huaihe River, as experimental material, this study investigated the effects of application of 0, 30, 60, 90 and 120 m3 x hm(-2) pig farm slurry (PS) at the wintering stage combined with the application of 0, 30, 60, 90 kg x hm(-2) urea at the heading stage on medium- and micro-element contents and quality of wheat. The results showed that the Ca, Mg and Fe contents of plant firstly decreased then increased with the extension of the growth period, but the Cu, Zn and Mn contents decreased continuously through the growth period. Ca, Mg, Cu and Zn reached the highest value at every growth stage except the jointing stage when applying 120 m3 x hm(-2) PS with 90 kg x hm(-2) urea. Fe and Mn reached the highest value at every growth stage when applying 120 m3 x hm(-2) PS with 90 kg x hm(-2) urea. The contents of Ca, Mg, Cu, Zn, Fe and Mn all increased with increasing PS application across the growth period. Regarding the quality of wheat, applying 90 m3 x hm(-2) PS with 60 kg x hm(-2) urea was the best fertilization pattern. Combined applications of anaerobically treated PS with N fertilizer could increase medium- and micro-element contents and quality of wheat. Generally, it was recommended to apply 60-120 m3 x hm(-2) PS at the wintering stage and 90 kg x hm(-2) urea at the heading stage under field conditions.
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