AI Article Synopsis
- - Ciguatera fish poisoning is a significant and challenging type of seafood poisoning, prompting research into fish toxin profiles using a method called LC/MS/MS with various fish species from different Pacific locations.
- - Fish like snappers and groupers from Okinawa showed specific toxin patterns, with CTX1B and other related toxins present, while red snapper and amberjack from other areas like Hawaii contained different types of ciguatoxins.
- - The study found that toxin profiles were mostly consistent within species across different locations in Okinawa over 20 years, and the new LC/MS/MS method proved to be reliable compared to traditional mouse bioassays, with preparations made for future toxin analysis.
Article Abstract
Ciguatera fish poisoning is the most extensive and difficult to control of the seafood poisonings. To facilitate monitoring of fish toxicity, toxin profiles were investigated by an LC/MS/MS method using 14 reference toxins on eight representative species of fish collected in four different areas of the Pacific. Snappers and groupers from Okinawa contained ciguatoxin-1B (CTX1B) and two deoxy congeners at variable but species-specific ratios, while red snapper, Lutjanus bohar, from Minamitorishima, and amberjack, Seriola dumerili, from Hawaii, contained both CTX1B-type and CTX3C-type toxins. Spotted knifejaw, Oplegnathus punctatus, from Okinawan waters, contained mainly CTX4A and CTX4B, but the same species caught at Miyazaki was contaminated primarily with the CTX3C-type toxins. Otherwise, the toxin profiles were consistently species-specific in fish collected from various locations around Okinawa over 20 years. The LC/MS/MS and mouse bioassay results agreed well, indicating the LC/MS/MS method is a promising alternative to the mouse bioassay. Pure CTX1B and CTX3C were prepared for use in future LC/MS/MS analysis.
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