AI Article Synopsis
- The study focuses on identifying new protein partners in Pseudomonas aeruginosa to better understand the biological processes involving a specific protein.
- Current partner genes for the protein of interest are largely unknown and dispersed in the genome, complicating the identification process.
- The researchers developed pan-genomic bacterial two-hybrid libraries as a method to discover new protein partners in P. aeruginosa.
Article Abstract
In Pseudomonas aeruginosa, identification of new partners of a protein of interest could give precious clues to decipher a biological process in which this protein is involved. However, genes encoding for partners of a protein of interest are unknown and frequently scattered throughout the genome. We describe herein the construction and the use of pan-genomic bacterial two-hybrid libraries to identify new partners of a protein of interest encoded by P. aeruginosa.
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| http://dx.doi.org/10.1007/978-1-4939-0473-0_19 | DOI Listing |
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