AI Article Synopsis
- The study aimed to detect Toxoplasma gondii DNA using a technique called loop-mediated isothermal amplification (LAMP).
- Researchers extracted DNA from T. gondii tachyzoites and designed four primers targeting specific regions of the B1 gene to perform the LAMP assay.
- Results indicated that LAMP successfully detected T. gondii with high specificity and sensitivity, able to identify as few as 2-3 tachyzoites per reaction, while yielding no positive results from other tested organisms.
Article Abstract
Objective: To detect Toxoplasma gondii DNA by loop-mediated isothermal amplification (LAMP).
Methods: DNA was extracted by phenol-chloroform extraction from T. gondii tachyzoites. Four primers which recognized 6 distinct regions on the B1 gene of T. gondii were designed and used for LAMP assay. To evaluate the specificity of the method, Plasmodium vivax, P. falciparum, Pneumocystis carinii, Schistosoma japonicum, and mouse leucocytes were used gs controls. The parasite extract (T. gondii) was 10-fold serially diluted for evaluating the sensitivity of LAMP, and was amplified by LAMP. LAMP results were read with naked eye and analyzed by electrophoresis.
Results: After LAMP reaction, positive amplification was observed with T. gondii, but no positive signal was noted for the negative controls in the study. The sensitivity of LAMP assay reached up to 2-3 T. gondii tachyzoites/ml per reaction.
Conclusion: LAMP assay shows proper specificity and sensitivity for the detection of T. gondii.
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