AI Article Synopsis
- Two cDNA clones related to synaptic vesicle proteins were isolated from an electric lobe library of Torpedo california using specific antibodies.
- Northern blot analysis detected an 8- to 9-kb RNA transcript in the electric lobe and spinal cord, but absent in the brain and other non-neuronal tissues.
- Antibodies against a fusion protein showed that the target protein (85- to 90-kD) is primarily found in microsomal membranes of neurons and is linked to post-Golgi vesicles, indicated by a specific staining pattern.
Article Abstract
Two homologous cDNA clones were isolated from a Torpedo california electric lobe lambda gt11 expression library using a polyclonal antiserum directed against proteins associated with synaptic vesicles. Northern blotting reveals an 8- to 9-kb transcript in the electric lobe and the spinal cord, but not in the brain or other non-neuronal tissues. Antibodies generated against a fusion protein synthesized in Escherichia coli reacted with a 85- to 90-kD species in the neurons of the electric lobe. The immunoreactivity is associated with microsomal membranes and can be extracted readily with high salt. Immunohistochemical studies demonstrated a sparse punctate staining pattern in the cell body which colocalized with a subpopulation of post-Golgi vesicles.
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| http://dx.doi.org/10.1089/dna.1989.8.555 | DOI Listing |
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