We evaluated a two-site immunoluminometric assay (AFP LIA-mat byk) for the alpha-fetoprotein (AFP) determination. The assay, involving two monoclonal antibodies which recognize two different epitopes of the AFP, is rapid (approximately 4 h), with a wide working range (0-600 IU/ml) and a good sensitivity of detection. It presents a good reproducibility (CV less than 10% for the total working range). The regression equation for the AFP LIA-mat (Y) and the AFP bridge Serono (X) was Y = 1.175X-2.27 (n = 95, r = 0.996). It didn't present hook effect and, when we assessed the linearity by assaying a high concentration of AFP, it gave a linear response down to 3.5 IU/ml.
Download full-text PDF |
Source |
|---|
Publication Analysis
Top Keywords
Similar Publications
Reference Ranges and Determinants of Thyroid Function and TSH Receptor Antibodies During Early Pregnancy in Nepal.
Clin Endocrinol (Oxf)
December 2024
Department of Internal Medicine, Academic Center for Thyroid Diseases, Erasmus University Medical Center, Rotterdam, The Netherlands.
Objective: Different definitions of thyroid dysfunction during pregnancy may lead to under or overtreatment. The aims of this study were to (1) define population-based pregnancy-specific reference ranges for thyroid dysfunction during early pregnancy in Nepal and assess the impact of antibody positivity, (2) quantify the diagnostic impact of population-based reference ranges compared with current practice and (3) assess the determinants of thyroid function and antibody positivity.
Methods: A total of 800 healthy pregnant women aged 20-40 years in the Bhaktapur municipality were included.
Sandwich immunoassay for adrenomedullin precursor and its practical application.
Sci Rep
November 2024
PAM Theragnostics GmbH, 16761, Hennigsdorf, Germany.
Adrenomedullin (ADM) is a multifaceted peptide hormone involved in numerous physiological processes, including vascular stability, vasodilation, angiogenesis, and anti-inflammatory responses. The processing of ADM results in several fragments, including midregional proadrenomedullin (MR-proADM), and glycine-extended ADM (ADM-Gly) and bioactive ADM (bio-ADM). MR-proADM, the stable ADM fragment, and bio-ADM, the active form of ADM with a short half-life, have been shown to be potent biomarkers in a variety of pathologies.
View Article and Find Full Text PDFSerum peroxiredoxin-4, a biomarker of oxidative stress, associates with new-onset chronic kidney disease: A population-based cohort study.
Redox Biol
November 2024
Department of Gastroenterology and Hepatology, University of Groningen, University Medical Center Groningen, Groningen, the Netherlands; The Henry D. Janowitz Division of Gastroenterology, Department of Medicine, Icahn School of Medicine at Mount Sinai, New York, NY, United States.
Background: Chronic Kidney Disease (CKD), is often detected late due to its asymptomatic nature in the early stage of the disease. Overproduction of reactive oxygen species contributes to various pathological processes through oxidative stress (OS), impacting on cellular structures and functions with previous studies suggesting a link between OS and CKD progression. This study investigated the association between serum peroxiredoxin-4 (Prx4), a biomarker of oxidative stress, and the development of CKD in the general population.
View Article and Find Full Text PDFNeurological Damage Measured by S-100b and Neuron-Specific Enolase in Patients Treated with Electroconvulsive Therapy.
Brain Sci
August 2024
Departamento de Psiquiatría, Instituto Nacional de Neurología y Neurocirugía "Manuel Velasco Suárez", Insurgentes Sur 3877, Col. La Fama, Ciudad de México C.P. 14269, Mexico.
Electroconvulsive therapy (ECT) is considered one of the most effective treatments for psychiatric disorders. ECT has proven effective in the treatment of depression, mania, catatonia and psychosis. It is presumed that seizures induced during ECT administration cause toxicity and potentially neuronal and glial cell death.
View Article and Find Full Text PDFAnalytical performance of free testosterone calculated by direct immunoluminometric method compared with the Vermeulen equation: results from a clinical series.
Hormones (Athens)
June 2024
Department of Medical, Surgical and Neurological Sciences, University of Siena, Viale Bracci 16, 53100, Siena, Italy.
Introduction: Testosterone (T) is a hormone that is crucial for primary and secondary sexual development in both males and females. Free testosterone (FT) represents the biologically active form of T, and its measurement is of great importance in clinical practice. While application of either equilibrium dialysis or ultrafiltration is considered to be the gold standard for FT assessment, these methods are expensive and not widely accessible.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!