Specific interactions of extracellular matrix proteins with cells and their adhesion to the substrate are important for cell growth. A nanopatterned latex-coated paper substrate previously shown to be an excellent substrate for cell adhesion and 2D growth was studied for directed immobilization of proteins. The nanostructured latex surface was formed by short-wavelength IR irradiation of a two-component latex coating consisting of a hydrophilic film-forming styrene butadiene acrylonitrile copolymer and hydrophobic polystyrene particles. The hydrophobic regions of the IR-treated latex coating showed strong adhesion of bovine serum albumin (cell repelling protein), fibronectin (cell adhesive protein) and streptavidin. Opposite to the IR-treated surface, fibronectin and streptavidin had a poor affinity toward the untreated pristine latex coating. Detailed characterization of the physicochemical surface properties of the latex-coated substrates revealed that the observed differences in protein affinity were mainly due to the presence or absence of the protein repelling polar and charged surface groups. The protein adsorption was assisted by hydrophobic (dehydration) interactions.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/j.colsurfb.2014.03.050 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Enhancing the Properties of Latex-Based Coatings with Carboxylated Cellulose Nanocrystals.
Biomacromolecules
December 2024
Department of Chemical Engineering, Smith Engineering, Queen's University, Kingston, Ontario K7L 3N6, Canada.
Latex-based nanocomposites containing carboxylated cellulose nanocrystals (cCNCs) were synthesized via in situ seeded semibatch emulsion polymerization. Inspired by nature's use of CNCs to enhance rigidity and mechanical strength in cellulosic materials, we explored similar principles to improve the properties of acrylate water-based coatings. The cCNCs, loaded at 0.
View Article and Find Full Text PDFMapping Structure and Rheology of pH-Responsive Resins for Low-VOC Coatings.
ACS Appl Mater Interfaces
December 2024
Department of Chemical and Biological Engineering, Northwestern University, Evanston, Illinois 60208, United States.
In recent years, the paint and coatings industry has shifted away from traditional resin formulations that require high concentrations of volatile organic compounds (VOCs) to achieve the desired rheological performance and sustainability targets. One approach to eliminate or reduce VOCs in paint and coating formulations while maintaining the final performance is to disperse stimuli-responsive polymer latex particles in water. The chemistry and architecture of these particles have been engineered such that the suspension rheology changes in response to the pH changes.
View Article and Find Full Text PDFImproving the Theranostic Potential of Magnetic Nanoparticles by Coating with Natural Rubber Latex for Ultrasound, Photoacoustic Imaging, and Magnetic Hyperthermia: An In Vitro Study.
Pharmaceutics
November 2024
Department of Physics, FFCLRP, University of São Paulo, Av. Bandeirantes 3900, Ribeirão Preto 14040-901, São Paulo, Brazil.
Background/objectives: Magnetic nanoparticles (MNPs) have gained attention in theranostics for their ability to combine diagnostic imaging and therapeutic capabilities in a single platform, enhancing targeted treatment and monitoring. Surface coatings are essential for stabilizing MNPs, improving biocompatibility, and preventing oxidation that could compromise their functionality. Natural rubber latex (NRL) offers a promising coating alternative due to its biocompatibility and stability-enhancing properties.
View Article and Find Full Text PDFBiobased Composites from Eugenol- and Coumarin-Derived Methacrylic Latex and Hemp Nanocellulose: Cross-Linking via [2 + 2] Photocycloaddition and Barrier Properties.
ACS Sustain Chem Eng
June 2024
Dipartimento Scienza Applicata e tecnologia, Politecnico di Torino, 10129 Torino, Italy.
Article Synopsis
- A novel biobased latex was created through a process called redox-initiated emulsion copolymerization, resulting in a stable copolymer latex with specific solid content and particle size.
- The copolymer exhibited a transition temperature and underwent cross-linking when exposed to UVA light, leading to increased insolubility in acetone.
- When combined with hemp-derived nanocellulose, the resulting composite films demonstrated improved barrier properties, significantly reducing water vapor transmission rates compared to the films made solely from nanocellulose.
A rapid point-of-care population-scale dipstick assay to identify and differentiate SARS-CoV-2 variants in COVID-19-positive patients.
Front Microbiol
October 2024
Functional Genomics Laboratory, Centre for Microbial Research, Translational Health Science and Technology Institute, Faridabad, India.
Delta and Omicron variants of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) are remarkably contagious, and have been recognized as variants of concern (VOC). The acquisition of spontaneous substitutions or insertion-deletion mutations (indels) in the spike protein-encoding gene substantially increases the binding affinity of the receptor binding domain (RBD)-hACE2 complex and upsurges the transmission of both variants. In this study, we analyzed thousands of genome sequences from 30 distinct SARS-CoV-2 variants, focusing on the unique nucleic acid signatures in the spike gene specific to the Delta and Omicron variants.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!