In the present study a well-characterized strain collection (n = 33) of Avibacterium species was investigated by matrix-assisted laser desorption ionization-time-of flight mass spectrometry (MALDI-TOF MS). The robustness of the currently available reference database (Bruker Biotyper 3.0) was tested to determine the degree of identification of these strains. Reproducible signal patterns were obtained from all strains. However, identification of most strains was only possible at genus level. Furthermore, two strains could not be identified by this method. Based on their protein spectra profiles, a MALDI main spectra dendrogram was created to determine their relationship. Most strains were closely related-for example, 26 strains formed cluster 1 including the type strains of Avibacterium volantium, Avibacterium gallinarum, Avibacterium endocarditidis and Avibacterium avium-while Avibacterium paragallinarum biovars 1 and 2 formed cluster 2 and, finally, strain 55000 remained on its own. The present MALDI-TOF MS results confirm recent findings that only certain isolates of Av. paragallinarum represent a well-defined species within the genus Avibacterium, making a taxonomic revision essential. To improve identification of Avibacterium at species level by MALDI-TOF MS, relevant reference strains were included in the newly created database and results are presented. In conclusion, Av. paragallinarum can be identified by MALDI/Biotyper and not the other species of the genus.
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http://dx.doi.org/10.1080/03079457.2014.916038 | DOI Listing |
Vet Res
January 2025
Institute of Animal Husbandry and Veterinary Medicine, Beijing Academy of Agriculture and Forestry Sciences, Beijing, China.
Glutathione reductase (GR) plays a pivotal role in managing oxidative stress, a process crucial for microbial virulence and adaptation, yet it has not been extensively explored in bacteria such as Avibacterium paragallinarum (Av. paragallinarum). This study examined the specific roles of GR in Av.
View Article and Find Full Text PDFVet Microbiol
December 2024
College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, China. Electronic address:
Infectious coryza is an acute respiratory disease in chickens caused by Avibacterium paragallinarum. Lipooligosaccharides (LOSs) and capsular polysaccharides are important components of Av. paragallinarum.
View Article and Find Full Text PDFBiochem Biophys Res Commun
December 2024
International Joint Research Center for Microbiology and Infectious Diseases, Wohua Biotech, 256600, Binzhou, Shandong, China. Electronic address:
16S rRNA gene sequence is the most common housekeeping genetic marker to study bacterial phylogeny and taxonomy. Therefore, 16S rRNA gene sequencing has the potential to identify novel bacteria and diagnose bacteria. This study compared 16S rRNA gene sequencing with conventional PCR for bacterial identification and disease diagnosis.
View Article and Find Full Text PDFAvian Dis
September 2024
The Pennsylvania State University, Veterinary and Biomedical Sciences, University Park, PA 16802.
Front Vet Sci
August 2024
Beijing Key Laboratory for Prevention and Control of Infectious Diseases in Livestock and Poultry, Institute of Animal Husbandry and Veterinary Medicine, Beijing Academy of Agriculture and Forestry Sciences, Beijing, China.
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