AI Article Synopsis
- The health of transplanted human aortic valves is closely linked to their cellular and immune status at the time of surgery.
- A new test using Alcian Blue dye measures how well endothelial cells are functioning by checking their integrity through nuclear uptake.
- The study found that as storage time and temperature increased, the integrity of the aortic valves' cells declined, while DMSO offered little benefit in preserving cell integrity.
Article Abstract
The fate of human allogeneic aortic valves depends mainly on their histological and immunological condition at the time of transplantation. A screening test making novel use of Alcian Blue was used to determine the integrity of endothelial cells as a prerequisite to their function. The dye uptake into the nucleus was measured quantitatively. The test was used to compare the effect of different storage mediums and temperatures (+4 degrees C, -30 degrees C, -80 degrees C, DMSO, FCS, RPMI, antibiotic solution) on aortic valves of rats. The cell integrity decreased with increasing storage time and higher storage temperature. The cryoprotective agent DMSO had no essential effect on the maintenance of cell integrity.
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| http://dx.doi.org/10.1055/s-2007-1020336 | DOI Listing |
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