AI Article Synopsis
- * Human skin keratinocytes use specific integrins to connect with the extracellular matrix (ECM) in healthy skin, making these interactions vital for proper cellular function.
- * Artificial ECM proteins, designed to mimic natural ECM, enable targeted cell responses and support the growth of skin stem cells, making them promising for skin tissue engineering applications.
Article Abstract
Cell-matrix interactions play critical roles in regulating cellular behavior in wound repair and regeneration of the human skin. In particular, human skin keratinocytes express several key integrins such as alpha5beta1, alpha3beta1, and alpha2beta1 for binding to the extracellular matrix (ECM) present in the basement membrane in uninjured skin. To mimic these key integrin-ECM interactions, artificial ECM (aECM) proteins containing functional domains derived from laminin 5, type IV collagen, fibronectin, and elastin are prepared. Human skin keratinocyte cell responses on the aECM proteins are specific to the cell-binding domain present in each construct. Keratinocyte attachment to the aECM protein substrates is also mediated by specific integrin-material interactions. In addition, the aECM proteins are able to support the proliferation of keratinocyte stem cells, demonstrating their promise for use in skin tissue engineering.
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| http://dx.doi.org/10.1002/mabi.201400015 | DOI Listing |
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