AI Article Synopsis
- - Mice with genetic modifications are increasingly used in heart research to model human diseases, but variations among different mouse strains necessitate standardized methods for cardiovascular analysis.
- - This study introduces non-invasive echocardiography and electrocardiography techniques for quickly assessing the heart function of awake mice in large-scale studies.
- - Three mouse strains (C57BL/6N, C57BL/6J, C3HeB/FeJ, and 129P2/OlaHsd) were investigated, revealing that C57BL/6N exhibits heart failure symptoms, while also identifying genetic influences on cardiac structure and function.
Article Abstract
Mice with genetic alterations are used in heart research as model systems of human diseases. In the last decade there was a marked increase in the recognition of genetic diversity within inbred mouse strains. Increasing numbers of inbred mouse strains and substrains and analytical variation of cardiac phenotyping methods require reproducible, high-throughput methods to standardize murine cardiovascular physiology. We describe methods for non-invasive, reliable, easy and fast to perform echocardiography and electrocardiography on awake mice. This method can be used for primary screening of the murine cardiovascular system in large-scale analysis. We provide insights into the physiological divergence of C57BL/6N, C57BL/6J, C3HeB/FeJ and 129P2/OlaHsd mouse hearts and define the expected normal values. Our report highlights that compared to the other three strains tested C57BL/6N hearts reveal features of heart failure such as hypertrophy and reduced contractile function. We found several features of the mouse ECG to be under genetic control and obtained several strain-specific differences in cardiac structure and function.
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| http://dx.doi.org/10.1007/s00360-014-0830-3 | DOI Listing |
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