AI Article Synopsis
- - The chapter outlines methods for studying cells that produce TNF using both lab-based (in vitro) and live organism (in vivo) techniques.
- - It introduces a new type of transgenic mouse that has been genetically engineered to produce a far-red fluorescent protein, making it easier to identify cells that produce TNF.
- - The design of these mice allows researchers to detect, isolate, and visualize TNF-producing cells due to the reporter protein being linked to the same regulatory mechanisms as TNF itself.
Article Abstract
This chapter provides protocols for in vitro and in vivo analysis of TNF-producing cells from a novel TNF reporter mouse. In these transgenic mice, genetic sequence encoding far-red reporter protein Katyushka (FRFPK) was placed under control of the same regulatory elements as TNF, thus providing the basis for detection, isolation, and visualization of TNF-producing cells.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1007/978-1-4939-0669-7_13 | DOI Listing |
Publication Analysis
Top Keywords
tnf-producing cells
8
experimental applications
4
applications tnf-reporter
4
tnf-reporter mice
4
mice far-red
4
far-red fluorescent
4
fluorescent label
4
label chapter
4
chapter protocols
4
protocols vitro
4
Similar Publications
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!