Capsular polysaccharide was isolated by the phenol-water extraction of Acinetobacter baumannii ACICU cells and studied by sugar analysis, partial acid hydrolysis, and 1D and 2D (1)H and (13)C NMR spectroscopy. The polysaccharide was found to contain 5,7-diacetamido-3,5,7,9-tetradeoxy-l-glycero-l-manno-non-2-ulosonic or di-N-acetylpseudaminic acid (Pse5Ac7Ac), and the following structure of the branched tetrasaccharide repeating unit was established: The genes present in the polysaccharide gene cluster of A. baumannii ACICU are appropriate to the structure established.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/j.carres.2014.04.002 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
[Analysis of the virulence and genetic differences of carbapenem-resistant epidemic clones].
Zhonghua Yu Fang Yi Xue Za Zhi
May 2024
State Key Laboratory of Pathogen and Biosecurity, State Key Laboratory of Pathogen and Biosecurity, Beijing 100071, China.
Article Synopsis
- The study assessed the virulence levels of carbapenem-resistant bacterial clones ST191, ST195, and ST208 using genomic sequencing on 233 strains collected over eight years.
- The infection survival model showed that ST195 had the highest average mortality rate at 53.0%, while ST191 had the lowest at 23.0%, with significant statistical differences between the groups.
- Analysis of virulence genes revealed that ST191 lacked certain virulence factors, like type VI secretion system-related genes, which may contribute to its lower mortality rates compared to ST195 and ST208.
Characterization of the diversity of type IV secretion system-encoding plasmids in .
Emerg Microbes Infect
December 2024
Department of Biochemistry, Microbiology and Immunology, University of Ottawa, Ottawa, Canada.
The multi-drug resistant pathogen has gained global attention as an important clinical challenge. Owing to its ability to survive on surfaces, its capacity for horizontal gene transfer, and its resistance to front-line antibiotics, has established itself as a successful pathogen. Bacterial conjugation is a central mechanism for pathogen evolution.
View Article and Find Full Text PDFStructure-function correlates of fibrinogen binding by adhesins critical in catheter-associated urinary tract infections.
Proc Natl Acad Sci U S A
January 2023
Department of Molecular Microbiology, Washington University School of Medicine, St Louis, MO 63110.
Multidrug-resistant infections are an urgent clinical problem and can cause difficult-to-treat nosocomial infections. During such infections, like catheter-associated urinary tract infections (CAUTI), rely on adhesive, extracellular fibers, called chaperone-usher pathway (CUP) pili for critical binding interactions. The uropathogenic strain, UPAB1, and the pan-European subclone II isolate, ACICU, use the CUP pili Abp1 and Abp2 (previously termed Cup and Prp, respectively) in tandem to establish CAUTIs, specifically to facilitate bacterial adherence and biofilm formation on the implanted catheter.
View Article and Find Full Text PDFAn image processing-based quantification of gram variability in Acinetobacter baumannii.
Microsc Res Tech
March 2023
Department of Engineering, Roma Tre University, Rome, Italy.
Gram staining differentiates bacteria as gram-positive and gram-negative, depending on their cell wall constituents, and coloring cells in violet and pink, respectively. Sometimes, a subpopulation of the same bacterial species assumes different colors, ranging from pink to violet, for reasons that are not completely understood yet. We analyze conventional brightfield images and use an automated pipeline to count pink and violet cells.
View Article and Find Full Text PDFColistin and Carbapenem-Resistant Aci46 in Thailand: Genome Analysis and Antibiotic Resistance Profiling.
Antibiotics (Basel)
August 2021
Department of Microbiology, Faculty of Science, Mahidol University, Bangkok 10400, Thailand.
Resistance to the last-line antibiotics against invasive Gram-negative bacterial infection is a rising concern in public health. Multidrug resistant (MDR) Aci46 can resist colistin and carbapenems with a minimum inhibitory concentration of 512 µg/mL as determined by microdilution method and shows no zone of inhibition by disk diffusion method. These phenotypic characteristics prompted us to further investigate the genotypic characteristics of Aci46.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!