AI Article Synopsis
- The study examined the inhibitory effects of chitosan solutions on the rice pathogen Acidovorax avenae subsp. avenae (Aaa) strain RS-1, showing that higher concentrations and longer incubation times increased antibacterial efficiency.
- Chitosan was found to disrupt the bacterial cell membrane, as evidenced by cell lysis observed under electron microscopy and measurements of cell membrane integrity.
- Additionally, chitosan significantly reduced bacterial biofilm formation and altered the expression of specific genes related to the pathogen's response, highlighting its potential as a control method for bacterial brown stripe in rice.
Article Abstract
Inhibitory effect and mode of action of chitosan solution against rice bacterial brown stripe pathogen Acidovorax avenae subsp. avenae (Aaa) strain RS-1 was examined in this study. Result from this study indicated that chitosan solutions at 0.10, 0.20, and 0.40mg/mL inhibited the in vitro growth of Aaa strain RS-1, and in general the inhibitory efficiency increased with the increase of both chitosan concentration and the incubation time. Antibacterial activity of chitosan in this study may be mainly due to the damage of cell membrane, which was evidenced by both the cell lysis observed by transmission electron microscopy, and the increased release of cell materials based on the measurement of cell membrane integrity. Furthermore, chitosan solutions at concentrations of 0.1, 0.2, and 0.4mg/mL markedly inhibited bacterial biofilm formation compared to the control, and the inhibitory effect increased with the increase of chitosan concentration. In addition, quantitative real-time PCR of the 10 secretion system related genes revealed the differential expression of genes in particular ompA/motB, emphasizing the importance of this gene in the response of Aaa strain RS-1 to chitosan stress. These results indicated that the antibacterial mode of action of chitosan may be mainly due to membrane disruption and lysis, reduction of biofilm formation, and gene expression change. Overall, the results clearly indicated that chitosan had the potential to control bacterial brown stripe of rice.
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| http://dx.doi.org/10.1016/j.carres.2014.02.025 | DOI Listing |
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