Sclerostin is a negative regulator of bone formation. Sclerostin monoclonal antibody (Scl-Ab) treatment promoted bone healing in various animal models. To further evaluate the healing efficiency of Scl-Ab in osteotomy healing, we investigated the time course effects of systemic administration of Scl-Ab on fracture repair in rat femoral osteotomy model. A total of 120 six-month-old male SD rats were subjected to transverse osteotomy at the right femur mid-shaft. Rats were treated with vehicle or Scl-Ab treatment for 3, 6, or 9 weeks. Fracture healing was evaluated by radiography, micro-CT, micro-CT based angiography, 4-point bending mechanical test and histological assessment. Scl-Ab treatment resulted in significantly higher total mineralized callus volume fraction, BMD and enhanced neovascularization. Histologically, Scl-Ab treatment resulted in a significant reduction in fracture callus cartilage at week 6 and increase in bone volume at week 9, associated with a greater proportion of newly formed bone area at week 6 and 9 by fluorescence microscopy. Mechanical testing showed significantly higher ultimate load in Scl-Ab treatment group at week 6 and 9. This study has demonstrated that Scl-Ab treatment enhanced bone healing in a rat femoral osteotomy model, as reflected in increased bone formation, bone mass and bone strength.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1002/jor.22636 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Effect of anti-sclerostin antibody on orthodontic tooth movement in ovariectomized rats.
Prog Orthod
November 2024
Institute for Innovation in Digital Healthcare (IIDH), Yonsei University Health System, Seoul, Korea.
Background: This study aimed to evaluate the effect of systemically administered anti-sclerostin antibodies (Anti-Scl Ab) on orthodontic tooth movements (OTM) in an ovariectomized rat.
Methods: Twenty-four 12-week-old female Sprague-Dawley rats were randomly divided into two groups: (1) ovariectomy (OVX) group, (2) ovariectomy + romosozumab (ROMO) group. OTM was performed 8 weeks after OVX.
Carbonate Apatite Honeycomb Scaffold-Based Drug Delivery System for Repairing Osteoporotic Bone Defects.
ACS Appl Mater Interfaces
September 2024
Department of Biomaterials, Faculty of Dental Science, Kyushu University, 3-1-1 Maidashi, Higashi-ku, Fukuoka 812-8582, Japan.
Osteoporotic bone defects are difficult to repair in elderly patients. This study aimed to repair osteoporotic bone defects using a combination of bone tissue engineering (BTE) and drug delivery systems (DDS). Herein, honeycomb granules (HCGs) composed of carbonate apatite microspheres were fabricated as BTE scaffolds.
View Article and Find Full Text PDFSclerostin antibody corrects periodontal disease in type 2 diabetic mice.
JCI Insight
July 2024
Department of Physiology and Cell Biology, University of Arkansas for Medical Sciences, Little Rock, Arkansas, USA.
Article Synopsis
- - Type 2 diabetes (T2D) causes severe periodontal damage in a mouse model, leading to issues like inflamed gums, weakened periodontal ligaments, and significant bone loss, largely influenced by increased levels of a protein called sclerostin.
- - Treatment with a sclerostin-neutralizing antibody (Scl-Ab) significantly improved bone mass and reduced bone loss in T2D mice by suppressing harmful osteoclasts and promoting beneficial osteoblasts.
- - Scl-Ab also encouraged the growth of periodontal ligament cells and reduced inflammation, highlighting its potential as a therapeutic option for T2D-related oral complications with minimal side effects.
A Case of Palmoplantar Keratoderma in the Constellation of Connective Tissue Diseases.
Cureus
March 2024
Radiodiagnosis, All India Institute of Medical Sciences, Nagpur, Nagpur, IND.
Overlap syndrome is a clinical challenge and brings together a wide range of treatment options for the treating physician. Addressing each and every complaint of the patient is crucial. A 50-year-old female patient presented with skin thickening, blackening, and hyperkeratosis; dysphagia; joint pain; features of myopathy; Raynaud's phenomenon; and dry mouth.
View Article and Find Full Text PDFInfluence of anti-sclerostin monoclonal antibody in the repair of post-extraction sockets of ovariectomized rats.
Arch Oral Biol
June 2024
Department of Diagnosis and Surgery, School of Dentistry at Araraquara, São Paulo State University-UNESP, Araraquara, SP, Brazil. Electronic address:
Article Synopsis
- - This study focused on how an anti-sclerostin monoclonal antibody (Scl-Ab) affects bone healing after tooth extraction in ovariectomized rats, which are often used to model osteoporosis.
- - The research involved three groups of rats: healthy controls, those with induced osteoporosis, and those with osteoporosis treated with Scl-Ab, with all groups undergoing a molar extraction after a period of treatment.
- - Results showed that while Scl-Ab treatment didn't significantly speed up bone formation, it did enhance the healing quality and collagen content in the post-extraction area compared to the osteoporosis-only group.
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!