A sandwich enzyme immunoassay test utilising a mouse monoclonal antibody has been adapted for the sensitive detection of the p30 prostatic antigen in semen and in postcoital vaginal swabs. In liquid semen, p30 was still detectable at a 1/1,000,000 dilution, and it could still be detected on vaginal swabs 24 h postcoitus.
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http://dx.doi.org/10.1016/s0015-7368(89)73257-6 | DOI Listing |
Front Public Health
January 2025
Department of Global Health, Emory Rollins School of Public Health, Atlanta, GA, United States.
Background: Orthohantaviruses (also known as hantaviruses) are pathogens, primarily transmitted by rodents, that can cause hemorrhagic fever with renal syndrome (HFRS). In endemic regions of Kazakhstan, no confirmed HFRS cases were detected between 2020 and 2022 raising concerns about detection. Estimate antibody seroprevalence for hantaviruses and identify associated risk factors among high-risk adults in western Kazakhstan in 2023.
View Article and Find Full Text PDFFront Cell Infect Microbiol
January 2025
Jiangsu Engineering Research Center of Biological Data Mining and Healthcare Transformation, Xuzhou Medical University, Xuzhou, Jiangsu, China.
Introduction: Brucellosis, a significant zoonotic infectious disease, poses a global health threat. Accurate and efficient diagnosis is crucial for prevention, control, and treatment of brucellosis. VirB proteins, components of the Type IV secretion system (T4SS) in , play a pivotal role in bacterial virulence and pathogenesis but have been understudied for their diagnostic potential.
View Article and Find Full Text PDFEuro Surveill
January 2025
Institute of Biology, Faculty of Sciences, University of Pécs, Pécs, Hungary.
BackgroundCrimean-Congo haemorrhagic fever (CCHF) is a severe illness characterised by fever, bleeding and high case-fatality rates. The disease is caused by CCHF virus (CCHFV), transmitted by ticks and infectious body fluids and tissues.AimAfter CCHF was diagnosed in three persons in 2023, we aimed to investigate the presence of antibodies against CCHFV in healthcare workers (HCW), sheep and goats, and of CCHFV in ticks, in an area in North Macedonia and characterise virus strains.
View Article and Find Full Text PDFBMC Pregnancy Childbirth
January 2025
Department of Obstetrics and Gynecology, National Clinical Research Center for Obstetrics and Gynecology, Peking University Third Hospital, Peking University Third Hospital), National Center for Healthcare Quality Management in Obstetrics, Beijing, 100191, China.
Background: Postpartum hemorrhage (PPH) is the leading cause of maternal mortality worldwide, with uterine atony accounting for approximately 70% of PPH cases. However, there is currently no effective prediction method to promote early management of PPH. In this study, we aimed to screen for potential predictive biomarkers for atonic PPH using combined omics approaches.
View Article and Find Full Text PDFAnal Chem
January 2025
State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Jilin, Changchun 130022, China.
Lateral flow immunochromatography (LFIA) with gold nanoparticles (AuNPs) is widely used in the biomedical field as a rapid and simple in vitro detection technique. However, the conventional AuNP-LFIA has limitations in sensitivity and detection range. In this study, nonprecious metal iron-based bimetallic FeWO nanomaterials with convenient and excellent enzyme-mimetic catalytic activities were synthesized by a one-pot hydrothermal method.
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